Lv Zhao, Hu Yazhou, Tan Jin, Wang Xiaoqing, Liu Xiaoyan, Zeng Cong
Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China.
School of Oceanography, Shanghai Jiao Tong University, Shanghai 200240, China.
Biology (Basel). 2021 Nov 22;10(11):1218. doi: 10.3390/biology10111218.
Although hemorrhagic sepsis caused by infection is the dominant disease in the aquaculture of Chinese soft-shelled turtle, information on its molecular pathology is seriously limited. In this study, ninety turtles intraperitoneally injected with exhibited two different phenotypes based on the pathological symptoms, referred to as active and inactive turtles. Comparative transcriptomes of liver and spleen from these two groups at 6, 24, and 72 h post-injection (hpi) were further analyzed. The results showed that cytokine-cytokine receptor interaction, PRRs mediated signaling pathway, apoptosis, and phagocytosis enriched in active and inactive turtles were significantly different. Pro-inflammatory cytokines, the TLR signaling pathway, NLR signaling pathway, and RLR signaling pathway mediating cytokine expression, and apoptosis-related genes, were significantly up-regulated in inactive turtles at the early stage (6 hpi). The significant up-regulation of phagocytosis-related genes occurred at 24 hpi in inactive turtles and relatively lagged behind those in active turtles. The anti-inflammatory cytokine, IL10, was significantly up-regulated during the tested periods (6, 24, and 72 hpi) in active turtles. These findings offer valuable information for the understanding of molecular immunopathogenesis after infection, and facilitate further investigations on strategies against hemorrhagic sepsis in Chinese soft-shelled turtle .
尽管由感染引起的出血性败血症是中华鳖养殖中的主要疾病，但其分子病理学信息严重有限。在本研究中，90只经腹腔注射的鳖根据病理症状表现出两种不同的表型，分别称为活跃型和不活跃型鳖。对这两组鳖在注射后6、24和72小时（hpi）的肝脏和脾脏进行比较转录组分析。结果表明，活跃型和不活跃型鳖中富集的细胞因子-细胞因子受体相互作用、模式识别受体（PRRs）介导的信号通路、凋亡和吞噬作用存在显著差异。在早期（6 hpi），不活跃型鳖中介导细胞因子表达的促炎细胞因子、Toll样受体（TLR）信号通路、NOD样受体（NLR）信号通路和视黄酸诱导基因（RLR）信号通路以及凋亡相关基因显著上调。不活跃型鳖中吞噬作用相关基因的显著上调发生在24 hpi，相对滞后于活跃型鳖。抗炎细胞因子白细胞介素10（IL10）在活跃型鳖的测试期间（6、24和72 hpi）显著上调。这些发现为理解感染后的分子免疫发病机制提供了有价值的信息，并有助于进一步研究针对中华鳖出血性败血症的防治策略。
