Fan Lanlan, Qu Xiaosheng, Yi Tao, Peng Yong, Jiang Manjing, Miao Jianhua, Xiao Peigen
School of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530001, China.
National Engineering Laboratory of Southwest Endangered Medicinal Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
Evid Based Complement Alternat Med. 2020 Jan 28;2020:3472578. doi: 10.1155/2020/3472578. eCollection 2020.
The flavonoid dihydromyricetin (DMY) is the main component of (Hand-Mazz) W. T. Wang (AG), a daily beverage and folk medicine used in Southern China to treat jaundice hepatitis, cold fever, and sore throat. Recently, DMY and AG were shown to have a beneficial effect on lipid metabolism disorder. However, the mechanisms of how DMY and AG protect the liver during lipid metabolism disorder remain unclear. In this study, we first analyzed the chemical compounds of AG by HPLC-DAD-ESI-IT-TOF-MS . Of the 31 compounds detected, 29 were identified based on previous results. Then, the effects of DMY and AG on high-fat diet hamster livers were studied and the metabolite levels and metabolic pathway activity of the liver were explored by H NMR metabolomics. Compared to the high-fat diet group, supplementation of AG and DMY attenuated the high-fat-induced increase in body weight, liver lipid deposition, serum triglycerides and total cholesterol levels, and normalized endogenous metabolite concentrations. PCA and PLS-DA score plots demonstrated that while the metabolic profiles of hamsters fed a high-fat diet supplemented with DMY or AG were both far from those of hamsters fed a normal diet or a high-fat diet alone, they were similar to each other. Our data suggest that the underlying mechanism of the protective effect of DMY and AG might be related to an attenuation of the deleterious effect of high-fat diet-induced hyperlipidemia on multiple metabolic pathways including amino acid metabolism, ketone body metabolism, energy metabolism, tricarboxylic acid cycle, and enhanced fatty acid oxidation.
黄酮类化合物二氢杨梅素（DMY）是显齿蛇葡萄（Hand-Mazz）W. T. Wang（AG）的主要成分，AG是中国南方用于治疗黄疸型肝炎、感冒发烧和喉咙痛的日常饮品及民间药物。最近，DMY和AG被证明对脂质代谢紊乱具有有益作用。然而，DMY和AG在脂质代谢紊乱期间保护肝脏的机制仍不清楚。在本研究中，我们首先通过HPLC-DAD-ESI-IT-TOF-MS分析了AG的化学成分。在检测到的31种化合物中，基于先前的结果鉴定出了29种。然后，研究了DMY和AG对高脂饮食仓鼠肝脏的影响，并通过1H NMR代谢组学探索了肝脏的代谢物水平和代谢途径活性。与高脂饮食组相比，补充AG和DMY可减轻高脂诱导的体重增加、肝脏脂质沉积、血清甘油三酯和总胆固醇水平，并使内源性代谢物浓度正常化。PCA和PLS-DA得分图表明，虽然喂食补充了DMY或AG的高脂饮食的仓鼠的代谢谱与喂食正常饮食或单独高脂饮食的仓鼠的代谢谱都相差甚远，但它们彼此相似。我们的数据表明，DMY和AG保护作用的潜在机制可能与减轻高脂饮食诱导的高脂血症对包括氨基酸代谢、酮体代谢、能量代谢、三羧酸循环在内的多种代谢途径的有害影响以及增强脂肪酸氧化有关。
