显齿蛇葡萄二氢杨梅素的抗氧化特性及作用机制

Antioxidation properties and mechanism of action of dihydromyricetin from Ampelopsis grossedentata.

作者信息

Zhang You-sheng, Ning Zheng-xiang, Yang Shu-zhen, Wu Hui

机构信息

College of Food and Biological Engineering, South China University of Science and Technology, Guangzhou 510641, China.

出版信息

Yao Xue Xue Bao. 2003 Apr;38(4):241-4.

PMID:12889119

Abstract

AIM

To assess the antioxidative properties and the mechanism of action of dihydromyricetin (DMY) from Ampelopsis grossedentata.

METHODS

The antioxidative properties of DMY were measured by scavenging 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and inhibiting lipid peroxidation induced by FeSO4-edetic acid in linoleic acid. The mechanism of antioxidative properties of DMY was tested by measuring the chelating activities of DMY for Fe2+ with ultraviolet spectrum (UV) method.

RESULTS

The specific absorption of DPPH radical solution at 517 nm was reduced 73.3%-91.5% when DMY was added into the reaction solution in the concentration range from 0.01% to 0.04%. DMY was shown to greatly inhibit the increase of lipid peroxidation (LPO) values in linolei acid system catalyzed by FeSO4-edetic acid. The reaction rates (A532.min-1) of lipid peroxidation were 0.0021-0.0004 in the concentration range from 0.01% to 0.04% and the inhibition activities of DMY was found to be in a concentration-dependent manner. The mechanism of antioxidative properties of DMY was chelating Fe2+ in the Fe(2+)-dependent lipid peroxidation system.

CONCLUSION

DMY showed great antioxidative effect and would be a good natural antioxidant.

摘要

目的

评估显齿蛇葡萄中二氢杨梅素（DMY）的抗氧化特性及其作用机制。

方法

通过清除1,1-二苯基-2-苦基肼自由基（DPPH）以及抑制亚油酸中硫酸亚铁-乙二胺四乙酸诱导的脂质过氧化来测定DMY的抗氧化特性。采用紫外光谱（UV）法测定DMY对Fe2+的螯合活性，以此来测试DMY抗氧化特性的作用机制。

结果

当在反应溶液中加入浓度范围为0.01%至0.04%的DMY时，DPPH自由基溶液在517nm处的吸光度降低了73.3% - 91.5%。结果表明，DMY能显著抑制硫酸亚铁-乙二胺四乙酸催化的亚油酸体系中脂质过氧化（LPO）值的升高。在浓度范围为0.01%至0.04%时，脂质过氧化的反应速率（A532.min-1）为0.0021 - 0.0004，且发现DMY的抑制活性呈浓度依赖性。DMY抗氧化特性的作用机制是在依赖Fe2+的脂质过氧化体系中螯合Fe2+。