Bai Shijie, Hou Gang
Deep Sea Science Division, Institute of Deep Sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan, China.
Guangdong Ocean University, Zhanjiang, Guangdong, China.
PeerJ. 2020 Feb 7;8:e8517. doi: 10.7717/peerj.8517. eCollection 2020.
Coral reefs are an important part of the ocean ecosystem and are a vital spawning ground for marine fish. Microorganisms are abundant in this environment and play a key role in the growth and development of host species. Many studies have investigated the microbial communities of fish with a focus on the intestinal microbiome of laboratory-reared adult fish. Little is known about the relationship between fish eggs and their microorganisms, especially as microbial communities relate to wild fish eggs in coral reefs. In this study, we analyzed the microbial communities of two species of coral fish eggs, and , using 16S rRNA gene amplicon sequencing technology. , and were the main bacterial genera associated with these fish eggs and are known to be bacteria with potentially pathogenic and spoilage effects. The microbial community structures of and eggs were separated based on the 30 most abundant operational taxonomic units (OTUs). Principal coordinate analysis (PCoA) and non-metric multidimensional scaling analysis (NMDS) further confirmed that the microbial communities of coral fish eggs differ by species, which may be due to host selection. A functional prediction of the microbial communities indicated that most of the microbial communities were chemoheterotrophic and involved in nitrogen cycling. Our results showed that the microbial communities of coral fish eggs were distinct by species and that key microorganisms were potentially pathogenic, leading to the spoilage of fish eggs, high mortality, and low incubation rates. This study provided new insights for understanding the relationship between microorganisms and wild fish eggs.
珊瑚礁是海洋生态系统的重要组成部分，是海洋鱼类至关重要的产卵地。微生物在这种环境中大量存在，并在宿主物种的生长和发育中发挥关键作用。许多研究调查了鱼类的微生物群落，重点是实验室饲养的成年鱼的肠道微生物组。关于鱼卵与其微生物之间的关系，尤其是与珊瑚礁中野生鱼卵相关的微生物群落，人们了解甚少。在本研究中，我们使用16S rRNA基因扩增子测序技术分析了两种珊瑚鱼卵的微生物群落。 、 和 是与这些鱼卵相关的主要细菌属，已知是具有潜在致病和腐败作用的细菌。 和 鱼卵的微生物群落结构基于30个最丰富的可操作分类单元（OTU）进行了区分。主坐标分析（PCoA）和非度量多维尺度分析（NMDS）进一步证实，珊瑚鱼卵的微生物群落因物种而异，这可能是由于宿主选择。对微生物群落的功能预测表明，大多数微生物群落是化能异养型的，并参与氮循环。我们的结果表明，珊瑚鱼卵的微生物群落因物种而异，关键微生物具有潜在致病性，导致鱼卵腐败、高死亡率和低孵化率。本研究为理解微生物与野生鱼卵之间的关系提供了新的见解。
