中国西南部宠物啮齿动物中某物种的首次亚型分类。
First subtyping of sp. from pet rodents in southwestern China.
作者信息
Chai Yijun, Deng Lei, Liu Haifeng, Yao Jingxin, Zhong Zhijun, Fu Hualin, Shen Liuhong, Zhou Ziyao, Deng Junliang, Hu Yanchun, Peng Guangneng
机构信息
The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
出版信息
Int J Parasitol Parasites Wildl. 2020 Jan 30;11:143-148. doi: 10.1016/j.ijppaw.2020.01.012. eCollection 2020 Apr.
sp. is a common eukaryotic parasite, which infects humans as well as various other animals. To date, epidemiological data regarding the detection rate and distribution of sp. subtypes in pet rodents are lacking in China; the present study aims to fill this gap. A total of 503 fecal samples collected from pets in different locations in southwestern China were screened for the presence of sp. using a nested PCR amplification of SSU rRNA method. Forty-two samples (8.35%) tested positive for sp. colonization. Two subtypes of sp. were identified based on nucleotide sequence homology and phylogenetic analysis: ST4 was present in 41 samples, and ST17 was found in 1 sample. Our results revealed robust host preference of ST4 and confirmed that ST17 can also parasitize rodents.
sp.是一种常见的真核寄生虫，可感染人类以及各种其他动物。迄今为止，中国缺乏关于宠物啮齿动物中sp.亚型的检出率和分布的流行病学数据；本研究旨在填补这一空白。使用SSU rRNA的巢式PCR扩增方法，对从中国西南部不同地点的宠物收集的总共503份粪便样本进行了sp.检测。42份样本（8.35%）检测出sp.定植呈阳性。基于核苷酸序列同源性和系统发育分析鉴定出sp.的两个亚型：41份样本中存在ST4，1份样本中发现ST17。我们的结果揭示了ST4对宿主有强烈的偏好，并证实ST17也可寄生于啮齿动物。
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