Wang Pei, Li Sen, Zou Yang, Hong Zhao-Wei, Wang Ping, Zhu Xing-Quan, Song De-Ping, Chen Xiao-Qing
Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Pathogens. 2021 Sep 14;10(9):1189. doi: 10.3390/pathogens10091189.
sp. is a common pathogen that infects the intestines of humans and animals, causing a threat to public health. However, little information on the prevalence and subtypes of sp. in diarrheic pigs in China is available. Herein, 1254 fecal samples were collected from diarrheic pigs in 37 intensive pig farms in Hunan, Jiangxi, and Fujian provinces in southern China, and the prevalence and subtypes of sp. were investigated. sp. was detected by PCR assay, which amplified the small subunit rRNA (SSU rRNA) gene. Overall prevalence of sp. was 31.4% (394/1254), including 21.5% (66/307), 33.1% (99/299), 58.9% (56/95), and 31.3% (173/553) in suckling piglets, weaned piglets, fattening pigs, and sows, respectively. Moreover, age and region factors were significantly related to prevalence of sp. ( < 0.05). Four sp. subtypes were identified, including ST1, ST3, ST5, and ST14. The preponderant subtype was ST5 (76.9%, 303/394). To our knowledge, ST14 was firstly found in pigs in China. The human-pathogenic subtypes (ST1, ST3, ST5, and ST14) that were observed in this study indicate a potential threat to public health. These findings provided a new sight for studying the genetic structure of sp.
某菌是一种常见病原体，可感染人类和动物肠道，对公共卫生构成威胁。然而，关于中国腹泻猪中该菌的流行情况和亚型的信息却很少。在此，从中国南方湖南、江西和福建三省37个集约化养猪场的腹泻猪中采集了1254份粪便样本，对该菌的流行情况和亚型进行了调查。通过PCR检测法检测该菌，该方法扩增小亚基rRNA（SSU rRNA）基因。该菌的总体流行率为31.4%（394/1254），其中哺乳仔猪、断奶仔猪、育肥猪和母猪中的流行率分别为21.5%（66/307）、33.1%（99/299）、58.9%（56/95）和31.3%（173/553）。此外，年龄和地区因素与该菌的流行率显著相关（P<0.05）。鉴定出四种该菌亚型，包括ST1、ST3、ST5和ST14。优势亚型为ST5（76.9%，303/394）。据我们所知，ST14首次在中国猪中发现。本研究中观察到的人致病亚型（ST1、ST3、ST5和ST14）表明对公共卫生存在潜在威胁。这些发现为研究该菌的遗传结构提供了新视角。
