Takemoto Kazunori, Doi Wakana, Masuoka Noriyoshi
Kake Medical Science Education Center, Okayama 700-0005, Japan.
Department of Life Science, Okayama University of Science, Okayama 700-0005, Japan.
Biochim Biophys Acta. 2016 Apr;1862(4):647-650. doi: 10.1016/j.bbadis.2015.12.022. Epub 2015 Dec 23.
Alloxan induces oxidative stress and hyperglycemia in animal models. Acatalasemic (catalase deficiency) mice are susceptible to alloxan-induced hyperglycemia. As the incidence of hyperglycemia induced by alloxan was reportedly improved when mice were fed a vitamin E supplemented diet, this protective effect was examined.
Acatalasemic and normal mice fed a vitamin E supplemented diet were treated with alloxan. The pancreas were examined with microscopy. We also isolated pancreatic islets of normal mice treated with alloxan. The glucose stimulated insulin secretion was examined.
Vitamin E powerfully ameliorated the increase in apoptosis. Vitamin E increases insulin amounts secreted from pancreatic cells, but does not ameliorate the regulation of the glucose stimulated insulin secretion.
It is suggested that the difference in the mice fed vitamin E supplemented diet is due to an increase of insulin secretion and that vitamin E supplementation may have a role in helping to slow the stages of diabetes mellitus.
四氧嘧啶可在动物模型中诱导氧化应激和高血糖。无过氧化氢酶血症（过氧化氢酶缺乏）小鼠易受四氧嘧啶诱导的高血糖影响。据报道，当给小鼠喂食补充维生素E的饮食时，四氧嘧啶诱导的高血糖发生率有所改善，因此对这种保护作用进行了研究。
给喂食补充维生素E饮食的无过氧化氢酶血症小鼠和正常小鼠注射四氧嘧啶。用显微镜检查胰腺。我们还分离了注射四氧嘧啶的正常小鼠的胰岛。检测葡萄糖刺激的胰岛素分泌。
维生素E有力地改善了细胞凋亡的增加。维生素E增加了胰腺细胞分泌的胰岛素量，但并未改善葡萄糖刺激的胰岛素分泌的调节。
提示喂食补充维生素E饮食的小鼠的差异是由于胰岛素分泌增加，并且补充维生素E可能在帮助减缓糖尿病进展阶段中起作用。
