Department of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, Shandong, People's Republic of China.
Department of Neurosurgery, Shandong Provincial Hospital, No.324, Jingwuweiqi Road, Jinan, 250021, Shandong, People's Republic of China.
Mol Biol Rep. 2020 Mar;47(3):2181-2187. doi: 10.1007/s11033-020-05317-6. Epub 2020 Feb 19.
Recent research have proved that miR-501-5p acted as a potent tumor biomarker in several cancers, excluding head and neck squamous cell carcinoma (HNSCC). The study intends to discover the potential function and mechanism of miR-501-5p in HNSCC. Data from TCGA database and qRT-PCR estimated the expression of miR-501-5p and Calcium activated Chloride Channel A4 (CLCA4). Cell proliferation, clone formation and transwell assays were performed to explore HNSCC cells biological behaviors. Luciferase assay was carried out to identify the interaction between miR-501-5p and CLCA4. miR-501-5p was profoundly up-regulated in HNSCC samples and promoted cells proliferation and metastasis. CLCA4, as a target of miR-501-5p, was connected with worse outcomes in HNSCC patients. Co-transfection assay proved that miR-501-5p/CLCA4 functioned as crucial regulators to affect HNSCC cells biological behaviors. Our study illustrated that miR-501-5p exhibited a tumor-promoting role on HNSCC by targeting CLCA4, providing a new insight for revealing the pathogenesis and treatment of HNSCC.
最近的研究证明，miR-501-5p 在几种癌症中充当着强有力的肿瘤标志物，除了头颈部鳞状细胞癌（HNSCC）。本研究旨在探索 miR-501-5p 在 HNSCC 中的潜在功能和机制。TCGA 数据库和 qRT-PCR 数据评估了 miR-501-5p 和钙激活氯离子通道 A4（CLCA4）的表达。通过细胞增殖、克隆形成和 Transwell 实验探索 HNSCC 细胞的生物学行为。通过荧光素酶报告基因实验鉴定 miR-501-5p 和 CLCA4 之间的相互作用。miR-501-5p 在 HNSCC 样本中显著上调，促进了细胞的增殖和转移。CLCA4 作为 miR-501-5p 的靶标，与 HNSCC 患者的不良预后相关。共转染实验证明 miR-501-5p/CLCA4 作为关键调节因子影响 HNSCC 细胞的生物学行为。我们的研究表明，miR-501-5p 通过靶向 CLCA4 在 HNSCC 中发挥促肿瘤作用，为揭示 HNSCC 的发病机制和治疗提供了新的视角。
