Department of Oncology, The Second People's Hospital of Lianyungang (The Oncology Hospital of Lianyungang), Lianyungang City, Jiangsu Province 222000, China.
Comput Math Methods Med. 2021 Oct 18;2021:5953881. doi: 10.1155/2021/5953881. eCollection 2021.
This study attempted to investigate possible molecular mechanism and role of miR-18a-5p in head and neck squamous cell carcinoma (HNSCC). Differential miRNAs and their possible targets were analyzed through TCGA database. By conducting qRT-PCR, miR-18a-5p was tested to be increased and SORBS2 was assessed to be downregulated in HNSCC cells. CCK-8, Transwell, and flow cytometry assays disclosed that miR-18a-5p facilitated HNSCC cell proliferation, migration, and invasion and repressed cell apoptosis. By dual-luciferase reporter gene assay, it was verified that miR-18a-5p had binding sites into SORBS2. Rescue experiments displayed that forced expression of SORBS2 restored the impact of miR-18a-5p overexpression on HNSCC cells. Collectively, our research preliminarily identified the promotion effect of miR-18a-5p/SORBS2 axis on malignant phenotypes of HNSCC cells. Our findings may provide a preclinical reference for HNSCC treatment.
本研究试图探讨 miR-18a-5p 在头颈部鳞状细胞癌（HNSCC）中的可能分子机制和作用。通过 TCGA 数据库分析差异表达的 miRNAs 及其可能的靶基因。通过 qRT-PCR 检测到 miR-18a-5p 在 HNSCC 细胞中表达增加，SORBS2 表达下调。CCK-8、Transwell 和流式细胞术实验表明，miR-18a-5p 促进 HNSCC 细胞增殖、迁移和侵袭，抑制细胞凋亡。双荧光素酶报告基因实验验证了 miR-18a-5p 与 SORBS2 有结合位点。挽救实验显示，SORBS2 的过表达恢复了 miR-18a-5p 过表达对 HNSCC 细胞的影响。综上所述，我们的研究初步确定了 miR-18a-5p/SORBS2 轴对 HNSCC 细胞恶性表型的促进作用。我们的发现可能为 HNSCC 的治疗提供临床前参考。
