Dental student, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
Department of Laboratory Diagnostics, University Hospital Center Zagreb, Zagreb, Croatia.
Aust Dent J. 2020 Sep;65(3):205-209. doi: 10.1111/adj.12753. Epub 2020 Mar 27.
Oral wounds inevitably come into contact with saliva which can affect the time needed for bleeding to stop. The influence of saliva can be non-specific, related to dilution of blood, and/or mediated by salivary factors that affect haemostasis directly. The aim of this study was to assess if mixing blood with an individual's saliva would affect the rate of its coagulation measured by global coagulation tests, prothrombin time (PT) and activated partial thromboplastin time (APTT).
The study included 30 healthy non-smoking volunteers. Paired blood and unstimulated saliva samples were obtained from each participant and PT and APTT were determined in blood, blood + saliva and blood + water mixtures. Coagulation tests were performed using the mechanical clot detection method.
PT was significantly longer in both blood + saliva and blood + water mixtures compared to blood alone. APTT was significantly longer only in blood + water mixture compared to blood.
Similarly prolonged PT in both mixtures suggests that both saliva and water prolong coagulation evenly due to their non-specific effect of blood dilution. The finding that APTT was significantly prolonged only when blood was mixed with water could indicate presence of tissue factor in saliva, however, in a concentration too low to influence the results of PT.
口腔伤口不可避免地会接触到唾液，这可能会影响止血所需的时间。唾液的影响可能是非特异性的，与血液稀释有关，和/或受直接影响止血的唾液因素介导。本研究旨在评估将血液与个体的唾液混合是否会影响通过全凝血试验、凝血酶原时间 (PT) 和活化部分凝血活酶时间 (APTT) 测量的血液凝固率。
该研究纳入了 30 名健康不吸烟的志愿者。从每位参与者中采集配对的血液和未刺激的唾液样本，并在血液、血液+唾液和血液+水混合物中测定 PT 和 APTT。凝血试验采用机械凝块检测法进行。
与单独的血液相比，血液+唾液和血液+水混合物中的 PT 明显延长。与单独的血液相比，仅在血液+水混合物中 APTT 明显延长。
两种混合物中 PT 同样延长表明唾液和水由于非特异性的血液稀释作用均匀地延长了凝血。只有当血液与水混合时 APTT 明显延长的发现表明唾液中存在组织因子，但浓度太低，不会影响 PT 的结果。
