Baumann U, Frank R, Blöcker H
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, FRG.
Biochem Biophys Res Commun. 1988 Dec 30;157(3):986-91. doi: 10.1016/s0006-291x(88)80971-9.
Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration a hairpin with five dG residues in the loop is adsorbed to affinity matrix. Comparison of elution temperatures of hairpin oligonucleotides with those of single-stranded oligoguanylic acids with length of the loop indicates, that smallest loop able to bind forms a triplet of base pairs.
对原始tRNA和mRNA前体的非酶识别假说进行了实验研究。分析了环中含有不同数量脱氧鸟苷残基的DNA发夹与化学固定的寡聚（dC）单链的结合能力。在低浓度Mg2+存在下，环中含有五个dG残基的发夹被吸附到亲和基质上。将发夹寡核苷酸与环长度相同的单链寡聚鸟苷酸的洗脱温度进行比较，结果表明，能够结合的最小环形成了一个三联体碱基对。
