Brahmajothi Mulugu V, Abou-Donia Mohamed B
Department of Pharmacology and Cancer Biology, Duke University Medical Center, PO Box 3813, 308 Research Drive, Durham, NC 27710.
Mil Med. 2020 Jan 7;185(Suppl 1):279-285. doi: 10.1093/milmed/usz321.
Posttraumatic stress disorder (PTSD) can develop during the aftermath of traumatic events. Although many are impacted by several stressors, nearly 3.6% suffer from PTSD in the United States with higher incidence reported in military service personnel. Any injury to the blood-brain barrier can ignite an array of biological signaling molecules in the immune-privileged brain parenchyma, which can disrupt the synaptic neural network, resulting in altered behavior.
In this preliminary study, we compared 20 PTSD veterans with age-matched healthy veterans to identify plasma levels of brain-specific protein markers using enzyme-linked immunosorbent assay/immunofluorometric sandwich assay for neurotrophic factors and neuropoietic cytokines, and catalytic activity of matrix metalloproteinase (MMP) by zymography.
We observed an increased level of glial fibrillary acidic protein, tumor necrosis factor-alpha, interleukin 6, and MMP2 and MMP9 but decreased level of brain-derived neurotrophic factor, nerve growth factor-beta, and negligible difference in astroglial marker S100 calcium-binding protein B compared to controls.
Identification of neural biomarkers is essential to understand the subclinical symptoms for the diagnosis PTSD, which may not be visible by magnetic resonance imaging (MRI/fMRI) and may take years to clinically manifest.
创伤后应激障碍（PTSD）可在创伤事件后发生。尽管许多人受到多种应激源的影响，但在美国，近3.6%的人患有PTSD，据报道，军事服务人员的发病率更高。血脑屏障的任何损伤都可在免疫特权的脑实质中引发一系列生物信号分子，这可能会破坏突触神经网络，导致行为改变。
在这项初步研究中，我们将20名患有创伤后应激障碍的退伍军人与年龄匹配的健康退伍军人进行比较，使用酶联免疫吸附测定/免疫荧光夹心测定法检测神经营养因子和神经生成细胞因子的血浆脑特异性蛋白标志物水平，并通过酶谱法检测基质金属蛋白酶（MMP）的催化活性。
与对照组相比，我们观察到胶质纤维酸性蛋白、肿瘤坏死因子-α、白细胞介素6以及MMP2和MMP9水平升高，但脑源性神经营养因子、神经生长因子-β水平降低，星形胶质细胞标志物S100钙结合蛋白B的差异可忽略不计。
识别神经生物标志物对于理解创伤后应激障碍诊断中的亚临床症状至关重要，这些症状磁共振成像（MRI/fMRI）可能无法检测到，且可能需要数年时间才会在临床上显现出来。
