Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 26, 80126 Napoli, Italy.
Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Avenida Republica 330, 8370186 Santiago, Chile.
Int J Mol Sci. 2020 Feb 14;21(4):1277. doi: 10.3390/ijms21041277.
, formerly known as , is a spore-forming bacterium considered as the most common cause of nosocomial infections in developed countries. The spore of is involved in the transmission of the pathogen and in its first interaction with the host; therefore, a therapeutic approach able to control spores would improve the clearance of the infection. The C-terminal (CTD) end of BclA2, a spore surface protein of responsible of the interaction with the host intestinal cells, was selected as a putative mucosal antigen. The BclA2 fragment, BclA2, was purified and used to nasally immunize mice both as a free protein and after adsorption to the spore of , a well-established mucosal delivery vehicle. While the adsorption to spores increased the in vitro stability of BclA2, in vivo both free and spore-adsorbed BclA2 were able to induce a similar, specific humoral immune response in a murine model. Although in the experimental conditions utilized the immune response was not protective, the induction of specific IgG indicates that free or spore-bound BclA2 could act as a putative mucosal antigen targeting spores.
，以前称为 ，是一种形成孢子的细菌，被认为是发达国家医院获得性感染最常见的原因。的孢子参与病原体的传播及其与宿主的初次相互作用；因此，一种能够控制 孢子的治疗方法将提高感染的清除率。BclA2 的 C 末端（CTD）末端，一种负责与宿主肠细胞相互作用的 孢子表面蛋白，被选为潜在的粘膜抗原。BclA2 片段 BclA2 被纯化并用于鼻腔免疫接种小鼠，既可以作为游离蛋白，也可以吸附到 孢子上，这是一种成熟的粘膜传递载体。虽然吸附到孢子上增加了 BclA2 的体外稳定性，但在体内，游离和吸附到孢子上的 BclA2 都能够在小鼠模型中诱导相似的特异性体液免疫反应。尽管在使用的实验条件下，免疫反应没有保护作用，但特异性 IgG 的诱导表明游离或孢子结合的 BclA2 可以作为一种潜在的粘膜抗原，靶向 孢子。
