The Key Laboratory of Advanced Energy Materials Chemistry (MOE), and TKL of Metal and Molecule-based Material Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
Hebei Normal University of Science & Technology, Hebei 066004, China.
Sensors (Basel). 2020 Feb 14;20(4):1024. doi: 10.3390/s20041024.
Glucose oxidase (GOx) based biosensors are commercialized and marketed for the high selectivity of GOx. Incorporation nanomaterials with GOx can increase the sensitivity performance. In this work, an enzyme glucose biosensor based on nanotubes was fabricated. By using Ti foil as a carrier, hydrogen titanate nanotubes (HTNTs), which present fine 3D structure with vast pores, were fabricated in-situ by the hydrothermal treatment. The multilayer nanotubes are open-ended with a diameter of 10 nm. Then glucose oxidase (GOx) was loaded on the nanotubes by cross-linking to form an electrode of the amperometric glucose biosensor (GOx/HTNTs/Ti electrode). The fabricated GOx/HTNTs/Ti electrode had a linear response to 1-10 mM glucose, and the response time was 1.5 s. The sensitivity of the biosensor was 1.541 A·mM·cm, and the detection limit (S/N = 3) was 59 M. Obtained results indicate that the in-situ fabrication and unique 3D structure of GOx/HTNTs/Ti electrode are beneficial for its sensitivity.
葡萄糖氧化酶（GOx）基生物传感器因其对 GOx 的高选择性而被商业化和推向市场。将纳米材料与 GOx 结合可以提高传感器的灵敏度性能。在这项工作中，我们制备了一种基于纳米管的酶葡萄糖生物传感器。通过使用钛箔作为载体，通过水热处理原位制备了具有大量孔的精细 3D 结构的钛酸钠纳米管（HTNTs）。多层纳米管为开放式，直径为 10nm。然后，通过交联将葡萄糖氧化酶（GOx）负载在纳米管上，形成安培型葡萄糖生物传感器（GOx/HTNTs/Ti 电极）的电极。所制备的 GOx/HTNTs/Ti 电极对 1-10mM 葡萄糖具有线性响应，响应时间为 1.5s。该生物传感器的灵敏度为 1.541A·mM·cm，检测限（S/N=3）为 59µM。结果表明，GOx/HTNTs/Ti 电极的原位制备和独特的 3D 结构有利于提高其灵敏度。
