CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience , National Center for Nanoscience and Technology of China , Beijing 100190 , China.
University of Chinese Academy of Sciences , Beijing 100049 , China.
ACS Appl Mater Interfaces. 2019 Jan 23;11(3):2647-2654. doi: 10.1021/acsami.8b03118. Epub 2018 Apr 30.
Graphdiyne (GDY) is a novel two-dimensional (2D) carbon allotrope with sp-hybridized carbon atoms and hexagonal rings. Because of its unique structure and electronic property, GDY was reported as a promising candidate applied in energy storage, catalysis, biosensing and so on. However, using GDY as a platform to immobilize metal ion or enzyme was still not reported. Here, we presented a GDY-based composite with dual-enzyme activity by immobilizing ferrous ion and glucose oxidase onto GDY sheet. GDY showed great adsorption capacity and maintained the high catalytic activity of ferrous ion. The ferrous ion preferred to adsorb in between the neighboring two C-C triple bonds of GDY with lower adsorption energy (-5.64 eV) if compared to graphene (-1.69 eV). Meanwhile, GDY exhibited the ability of adsorbing glucose oxidase while did not obviously influence the structure and catalytic activity of the enzyme. The as-prepared composite was successfully used in one-step blood glucose detection. This work provides a new insight on ion and enzyme immobilization by 2D material.
二维（2D）碳同素异形体石墨炔（GDY）具有 sp 杂化碳原子和六元环。由于其独特的结构和电子特性，GDY 被报道为一种很有前途的储能、催化、生物传感等领域的候选材料。然而，将 GDY 用作固定金属离子或酶的平台的报道还没有。在这里，我们通过将亚铁离子和葡萄糖氧化酶固定在 GDY 片上，提出了一种具有双酶活性的 GDY 基复合材料。GDY 表现出了很强的吸附能力，并保持了亚铁离子的高催化活性。与石墨烯（-1.69 eV）相比，亚铁离子更喜欢吸附在 GDY 的相邻两个 C-C 三键之间，其吸附能较低（-5.64 eV）。同时，GDY 表现出吸附葡萄糖氧化酶的能力，而不会明显影响酶的结构和催化活性。所制备的复合材料成功地用于一步法血糖检测。这项工作为二维材料固定离子和酶提供了新的见解。
