Prasad Pawar Rajendra, Mishra Pooja, Durgbanshi Abhilasha, Bose Devasish, Albiol-Chiva Jaume, Peris-Vicente Juan, García-Ferrer Daniel, Esteve-Romero Josep
Department of Criminology and Forensic Science, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Sagar, Madhya Pradesh 470003, India.
Department of Chemistry, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Sagar, Madhya Pradesh 470003, India.
Antibiotics (Basel). 2020 Feb 14;9(2):86. doi: 10.3390/antibiotics9020086.
Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)-6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25-50 ppm), sensitivity (limit of detection 0.1-0.2 ppm; limit of quantification, 0.3-0.6 ppm), trueness (92.5-102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control.
甲苯咪唑是一种用于养牛业的驱虫药。然而，其残留物可能会出现在生产的食品和排泄物中，危害公众健康。开发了一种基于胶束液相色谱法（MLC）的方法，用于测定乳制品（牛奶、奶酪、黄油和凝乳）以及牛的含氮排泄物（尿液和粪便）中的甲苯咪唑。样品处理简化为简单稀释或固液萃取，然后过滤并直接进样所得溶液。使用C18柱和由0.15 M十二烷基硫酸钠（SDS）-6% 1-戊醇组成、pH值为7的磷酸盐缓冲流动相，在等度模式下以1 mL/min的流速运行，在不到8分钟的时间内将分析物与基质化合物分离。通过在292 nm处的吸光度进行检测。该方法根据欧盟委员会第2002/657/EC号决定的指导方针进行了验证，验证内容包括：特异性、方法校准范围（从定量限到25 - 50 ppm）、灵敏度（检测限0.1 - 0.2 ppm；定量限0.3 - 0.6 ppm）、准确性（92.5 - 102.3%）、精密度（<7.5%，以相对标准偏差表示）、稳健性和稳定性。该方法可靠、灵敏、易于操作、环保、安全、廉价且具有高样品通量。因此，作为实验室药物残留控制的筛查或定量方法，它对于常规分析很有用。
