Desmarchelier Aurélien, Fan Kaïli, Minh Tien Mai, Savoy Marie-Claude, Tarres Adrienne, Fuger Denis, Goyon Alexandre, Bessaire Thomas, Mottier Pascal
a Nestlé Research Center , Institute of Food Safety and Analytical Science , Lausanne , Switzerland.
b Nestlé Quality Assurance Center , Singapore.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018 Apr;35(4):646-660. doi: 10.1080/19440049.2018.1429677. Epub 2018 Feb 20.
A procedure for screening 105 veterinary drugs in foods by liquid chromatography tandem mass-spectrometry (LC-MS/MS) is presented. Its scope encompasses raw materials of animal origin (milk, meat, fish, egg and fat) but also related processed ingredients and finished products commonly used and manufactured by food business operators. Due to the complexity of the matrices considered and to efficiently deal with losses during extraction and matrix effects during MS source ionisation, each sample was analysed twice, that is 'unspiked' and 'spiked at the screening target concentration' using a QuEChERS-like extraction. The entire procedure was validated according to the European Community Reference Laboratories Residues Guidelines. False-negative and false-positive rates were below 5% for all veterinary drugs whatever the food matrix. Effectiveness of the procedure was further demonstrated through participation to five proficiency tests and its ruggedness demonstrated in quality control operations by a second laboratory.
本文介绍了一种通过液相色谱串联质谱法（LC-MS/MS）筛查食品中105种兽药的方法。其范围涵盖动物源性原料（牛奶、肉类、鱼类、蛋类和脂肪），以及食品经营商常用和生产的相关加工配料和成品。由于所考虑基质的复杂性，以及为有效处理提取过程中的损失和质谱源电离过程中的基质效应，每个样品都使用类似QuEChERS的提取方法进行了两次分析，即“未加标”和“以筛查目标浓度加标”。整个程序根据欧洲共同体参考实验室残留指南进行了验证。无论食品基质如何，所有兽药的假阴性率和假阳性率均低于5%。通过参加五次能力验证测试进一步证明了该方法的有效性，并且第二个实验室在质量控制操作中证明了该方法的耐用性。
