Facultad de Agronomía y Veterinaria, Universidad Autónoma de San Luis Potosí, Potosí, Mexico.
Laboratorio de Microbiología Molecular, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico.
Arch Microbiol. 2020 Jul;202(5):1173-1179. doi: 10.1007/s00203-020-01828-9. Epub 2020 Feb 19.
Escherichia coli strains are part of the normal biota of humans and animals; however, several clinical reports have implicated E. coli as the etiological agent of diarrhea in humans and companion animals. Thus, the aim of the present study was to know if companion dogs in the city of San Luis Potosi are colonized with virulent potentially harmful E. coli strains. Rectal swabs from 30 dogs, 13 with and 17 without diarrhea were analyzed. Phylogenetic and virulence genes analysis was performed to the E. coli isolates. Additionally, the Kirby-Bauer test was used to analyze the sensitivity to 32 different antimicrobials from 14 families. Eighty-five isolates were identified as E. coli and detected in 97% of healthy and diarrheic dog samples. E. coli isolates from healthy dogs carried several virulence genes, in contrast with those from diarrheic animals that presented only eaeA. In healthy dogs, phylogenetic analysis showed that 57% and 43% of E. coli isolates belonged to commensal (A and B1) and virulent (B2 and D) groups respectively. Meanwhile, diarrheic dogs showed that 69% of the isolates were identified as virulent B2 and D phylogroups. Moreover, E. coli resistant to β-lactams, aminoglycosides, tetracycline, quinolones, and folate inhibitors were detected in both groups of dogs. The presence of E. coli with eaeA virulence gene in diarrheic dogs, suggest that these strains are associated with the animal´s condition. Finally, major attention must be drawn to the careful handling of dogs because of their capability to harbor and disseminate virulent E. coli strains.
大肠杆菌菌株是人类和动物正常生物群的一部分；然而，有几项临床报告表明大肠杆菌是人类和伴侣动物腹泻的病原体。因此，本研究旨在了解圣路易斯波托西市的伴侣犬是否定植有潜在有害的毒力大肠杆菌菌株。分析了 30 只狗的直肠拭子，其中 13 只有腹泻，17 只没有腹泻。对大肠杆菌分离株进行了系统发育和毒力基因分析。此外，还使用 Kirby-Bauer 试验分析了来自 14 个家族的 32 种不同抗菌药物的敏感性。鉴定出 85 株为大肠杆菌，在健康和腹泻犬样本中的检出率为 97%。与腹泻动物只携带 eaeA 基因的情况相反，健康犬的大肠杆菌分离株携带多种毒力基因。在健康犬中，系统发育分析显示，57%和 43%的大肠杆菌分离株分别属于共生（A 和 B1）和毒力（B2 和 D）组。而腹泻犬的分离株中有 69%鉴定为毒力 B2 和 D 菌系。此外，在两组犬中均检测到对β-内酰胺类、氨基糖苷类、四环素类、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。腹泻犬中存在携带 eaeA 毒力基因的大肠杆菌，提示这些菌株与动物的状况有关。最后，必须引起人们对狗的谨慎处理的重视，因为它们有能力携带和传播毒力大肠杆菌菌株。
