Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), C. Agustín Escardino 7, 46980, Valencia, Paterna, Spain.
Probiotics Antimicrob Proteins. 2020 Dec;12(4):1484-1491. doi: 10.1007/s12602-020-09639-6.
Inorganic arsenic [iAs, As(III) + As(V)] is considered a human carcinogen. Recent studies show that it has also toxic effects on the intestinal epithelium which might partly explain its systemic toxicity. The aim of this study is to evaluate the protective role of lactic acid bacteria (LAB) against the toxic effects of iAs on the intestinal epithelium. For this purpose, the human colonic cells Caco-2 were exposed to As(III) in the presence of various LAB strains or their conditioned medium. Results showed that some strains and their conditioned media partially revert the oxidative stress, the production of pro-inflammatory cytokines, the alterations of the distribution of tight junction proteins, and the cell permeability increases caused by As(III). These results show that both soluble factors secreted or resulting from LAB metabolism and cell-cell interactions are possibly involved in the beneficial effects. Therefore, some LAB strains have potential as protective agents against iAs intestinal barrier disruption.
无机砷[iAs，As(III) + As(V)]被认为是一种人类致癌物。最近的研究表明，它对肠道上皮也有毒性作用，这可能部分解释了其全身毒性。本研究旨在评估乳酸菌（LAB）对 iAs 对肠道上皮毒性的保护作用。为此，将人结肠细胞 Caco-2 暴露于 As(III)存在的各种 LAB 菌株或其条件培养基中。结果表明，一些菌株及其条件培养基部分逆转了由 As(III)引起的氧化应激、促炎细胞因子的产生、紧密连接蛋白分布的改变以及细胞通透性的增加。这些结果表明，由 LAB 代谢和细胞-细胞相互作用产生的可溶性因子可能参与了这种有益作用。因此，一些 LAB 菌株具有作为保护剂对抗 iAs 肠道屏障破坏的潜力。
