• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蜱唾液蛋白 Evasin-3 通过与激活的内皮细胞上沉积的 CXC 型趋化因子相互作用，可实现动脉炎症的可视化。

Tick Saliva Protein Evasin-3 Allows for Visualization of Inflammation in Arteries through Interactions with CXC-Type Chemokines Deposited on Activated Endothelium.

机构信息

Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstraße 8a, 80336, Munich, Germany.

出版信息

Bioconjug Chem. 2020 Mar 18;31(3):948-955. doi: 10.1021/acs.bioconjchem.0c00095. Epub 2020 Mar 4.

DOI:10.1021/acs.bioconjchem.0c00095
PMID:32077689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7086393/
Abstract

Atherosclerosis is one of the leading causes of mortality in developed and developing countries. The onset of atherosclerosis development is accompanied by overexpression of several inflammatory chemokines. Neutralization of these chemokines by chemokine-binding agents attenuates atherosclerosis progression. Here, we studied structural binding features of the tick protein Evasin-3 to chemokine (C-X-C motif) ligand (CXCL1). We showed that Evasin-3-bound CXCL1 is unable to activate the CXCR2 receptor, but retains affinity to glycosaminoglycans. This observation was exploited to detect inflammation by visualizing a group of closely related CXC-type chemokines deposited on cell walls in human endothelial cells and murine carotid arteries by a fluorescent Evasin-3 conjugate. This work highlights the applicability of tick-derived chemokine-binding conjugates as a platform for the development of new agents for inflammation imaging.

摘要

动脉粥样硬化是发达国家和发展中国家主要的死亡原因之一。动脉粥样硬化发展的发生伴随着几种炎症趋化因子的过度表达。趋化因子结合剂中和这些趋化因子可减轻动脉粥样硬化的进展。在这里，我们研究了蜱蛋白 Evasin-3 与趋化因子（C-X-C 基序）配体（CXCL1）的结构结合特征。我们表明，Evasin-3 结合的 CXCL1 无法激活 CXCR2 受体，但保留与糖胺聚糖的亲和力。这一观察结果被利用来通过荧光 Evasin-3 缀合物可视化在人内皮细胞和鼠颈动脉壁上沉积的一组密切相关的 CXC 型趋化因子，从而检测炎症。这项工作强调了蜱衍生趋化因子结合物作为开发新的炎症成像剂的平台的适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/8c4219ee3cce/bc0c00095_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/bf549e481ed4/bc0c00095_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/2997a51886ad/bc0c00095_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/695e02a2362c/bc0c00095_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/704f88b517f6/bc0c00095_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/d86c7eebedab/bc0c00095_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/8c4219ee3cce/bc0c00095_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/bf549e481ed4/bc0c00095_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/2997a51886ad/bc0c00095_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/695e02a2362c/bc0c00095_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/704f88b517f6/bc0c00095_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/d86c7eebedab/bc0c00095_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa40/7086393/8c4219ee3cce/bc0c00095_0006.jpg

相似文献

1
Tick Saliva Protein Evasin-3 Allows for Visualization of Inflammation in Arteries through Interactions with CXC-Type Chemokines Deposited on Activated Endothelium.蜱唾液蛋白 Evasin-3 通过与激活的内皮细胞上沉积的 CXC 型趋化因子相互作用，可实现动脉炎症的可视化。
Bioconjug Chem. 2020 Mar 18;31(3):948-955. doi: 10.1021/acs.bioconjchem.0c00095. Epub 2020 Mar 4.
2
Tick saliva protein Evasin-3 modulates chemotaxis by disrupting CXCL8 interactions with glycosaminoglycans and CXCR2.蜱唾液蛋白 Evasin-3 通过破坏 CXCL8 与糖胺聚糖和 CXCR2 的相互作用来调节趋化性。
J Biol Chem. 2019 Aug 16;294(33):12370-12379. doi: 10.1074/jbc.RA119.008902. Epub 2019 Jun 24.
3
Phylogenetic Analysis Indicates That Evasin-Like Proteins of Ixodid Ticks Fall Into Three Distinct Classes.系统发育分析表明，硬蜱属的 Evasin 样蛋白可分为 3 个不同的类。
Front Cell Infect Microbiol. 2021 Oct 22;11:769542. doi: 10.3389/fcimb.2021.769542. eCollection 2021.
4
A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.一个 knottin 支架决定了蜱抗独特型 evasins 对 CXC-趋化因子的结合特异性。
J Biol Chem. 2019 Jul 19;294(29):11199-11212. doi: 10.1074/jbc.RA119.008817. Epub 2019 Jun 5.
5
The N-terminal domain of a tick evasin is critical for chemokine binding and neutralization and confers specific binding activity to other evasins.蜱 evasin 的 N 端结构域对于趋化因子结合和中和至关重要，并赋予其他 evasin 特定的结合活性。
J Biol Chem. 2018 Apr 20;293(16):6134-6146. doi: 10.1074/jbc.RA117.000487. Epub 2018 Feb 27.
6
Structure-guided engineering of tick evasins for targeting chemokines in inflammatory diseases.基于结构的蜱抗独特型抗体工程改造用于靶向炎症性疾病中的趋化因子。
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). doi: 10.1073/pnas.2122105119.
7
Structural basis of chemokine recognition by the class A3 tick evasin EVA-ACA1001.A3 类蜱 evasin EVA-ACA1001 识别趋化因子的结构基础。
Protein Sci. 2024 Jun;33(6):e4999. doi: 10.1002/pro.4999.
8
Semisynthesis of an evasin from tick saliva reveals a critical role of tyrosine sulfation for chemokine binding and inhibition.从蜱唾液中半合成逃逸蛋白揭示了酪氨酸硫酸化在趋化因子结合和抑制中的关键作用。
Proc Natl Acad Sci U S A. 2020 Jun 9;117(23):12657-12664. doi: 10.1073/pnas.2000605117. Epub 2020 May 27.
9
Treatment with Evasin-3 reduces atherosclerotic vulnerability for ischemic stroke, but not brain injury in mice.Evasin-3 治疗可降低小鼠缺血性中风的动脉粥样硬化易损性，但不能减轻脑损伤。
J Cereb Blood Flow Metab. 2013 Apr;33(4):490-8. doi: 10.1038/jcbfm.2012.198. Epub 2012 Dec 19.
10
Platelets from flowing blood attach to the inflammatory chemokine CXCL16 expressed in the endothelium of the human vessel wall.来自流动血液中的血小板会附着在人血管壁内皮中表达的炎性趋化因子CXCL16上。
Thromb Haemost. 2015 Aug;114(2):297-312. doi: 10.1160/TH14-11-0911. Epub 2015 Apr 23.

引用本文的文献

1
Chemokines, molecular drivers of thromboinflammation and immunothrombosis.趋化因子：血栓炎症和免疫血栓形成的分子驱动因素。
Front Immunol. 2023 Oct 26;14:1276353. doi: 10.3389/fimmu.2023.1276353. eCollection 2023.
2
Chemokine Heteromers and Their Impact on Cellular Function-A Conceptual Framework.趋化因子异源二聚体及其对细胞功能的影响——概念框架。
Int J Mol Sci. 2023 Jun 30;24(13):10925. doi: 10.3390/ijms241310925.
3
The Importance of CXCL1 in Physiology and Noncancerous Diseases of Bone, Bone Marrow, Muscle and the Nervous System.

本文引用的文献

1
Structural basis of chemokine interactions with heparan sulfate, chondroitin sulfate, and dermatan sulfate.化学趋化因子与肝素硫酸、软骨素硫酸和硫酸皮肤素相互作用的结构基础。
J Biol Chem. 2019 Oct 25;294(43):15650-15661. doi: 10.1074/jbc.RA119.009879. Epub 2019 Aug 27.
2
Tick saliva protein Evasin-3 modulates chemotaxis by disrupting CXCL8 interactions with glycosaminoglycans and CXCR2.蜱唾液蛋白 Evasin-3 通过破坏 CXCL8 与糖胺聚糖和 CXCR2 的相互作用来调节趋化性。
J Biol Chem. 2019 Aug 16;294(33):12370-12379. doi: 10.1074/jbc.RA119.008902. Epub 2019 Jun 24.
3
A knottin scaffold directs the CXC-chemokine-binding specificity of tick evasins.
CXCL1 在骨骼、骨髓、肌肉和神经系统的生理学和非癌症疾病中的重要性。
Int J Mol Sci. 2022 Apr 11;23(8):4205. doi: 10.3390/ijms23084205.
4
Immunomodulatory Proteins in Tick Saliva From a Structural Perspective.从结构角度看蜱唾液中的免疫调节蛋白
Front Cell Infect Microbiol. 2021 Oct 13;11:769574. doi: 10.3389/fcimb.2021.769574. eCollection 2021.
5
Inhibition of platelet adhesion, thrombus formation, and fibrin formation by a potent αIIbβ3 integrin inhibitor from ticks.蜱源强效αIIbβ3整合素抑制剂对血小板黏附、血栓形成和纤维蛋白形成的抑制作用
Res Pract Thromb Haemost. 2020 Dec 18;5(1):231-242. doi: 10.1002/rth2.12466. eCollection 2021 Jan.
一个 knottin 支架决定了蜱抗独特型 evasins 对 CXC-趋化因子的结合特异性。
J Biol Chem. 2019 Jul 19;294(29):11199-11212. doi: 10.1074/jbc.RA119.008817. Epub 2019 Jun 5.
4
Chemokines from a Structural Perspective.从结构角度看趋化因子。
Int J Mol Sci. 2017 Oct 2;18(10):2088. doi: 10.3390/ijms18102088.
5
Ticks from diverse genera encode chemokine-inhibitory evasin proteins.来自不同属的蜱虫编码趋化因子抑制蛋白evasin。
J Biol Chem. 2017 Sep 22;292(38):15670-15680. doi: 10.1074/jbc.M117.807255. Epub 2017 Aug 4.
6
CXCL1/MGSA Is a Novel Glycosaminoglycan (GAG)-binding Chemokine: STRUCTURAL EVIDENCE FOR TWO DISTINCT NON-OVERLAPPING BINDING DOMAINS.CXCL1/MGSA是一种新型的糖胺聚糖（GAG）结合趋化因子：两个不同且不重叠结合域的结构证据。
J Biol Chem. 2016 Feb 19;291(8):4247-55. doi: 10.1074/jbc.M115.697888. Epub 2015 Dec 31.
7
Solution NMR characterization of chemokine CXCL8/IL-8 monomer and dimer binding to glycosaminoglycans: structural plasticity mediates differential binding interactions.趋化因子CXCL8/IL-8单体和二聚体与糖胺聚糖结合的溶液核磁共振表征：结构可塑性介导不同的结合相互作用。
Biochem J. 2015 Nov 15;472(1):121-33. doi: 10.1042/BJ20150059. Epub 2015 Sep 14.
8
Targeting chemokines: Pathogens can, why can't we?靶向趋化因子：病原体能够做到，我们为何不能？
Cytokine. 2015 Aug;74(2):259-67. doi: 10.1016/j.cyto.2015.02.011. Epub 2015 Mar 6.
9
Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013.全球、地区和国家按年龄、性别划分的 240 种死因的全死因和特定死因死亡率，1990-2013 年：2013 年全球疾病负担研究的系统分析。
Lancet. 2015 Jan 10;385(9963):117-71. doi: 10.1016/S0140-6736(14)61682-2. Epub 2014 Dec 18.
10
CXCL1 promotes arteriogenesis through enhanced monocyte recruitment into the peri-collateral space.CXCL1 通过增强单核细胞向侧支周围间隙的募集来促进血管生成。
Angiogenesis. 2015 Apr;18(2):163-71. doi: 10.1007/s10456-014-9454-1. Epub 2014 Dec 10.