Li Jialu, Liu Lingbin, Zhang Jipan, Cheng Lei, Ren Lingtong, Zhao Yongju
College of Animal Science and Technology, Southwest University, Beibei, Chongqing, P. R. China.
Chongqing Key Laboratory of Forage and Herbivore, Beibei, Chongqing, P. R. China.
Anim Biotechnol. 2021 Oct;32(5):573-579. doi: 10.1080/10495398.2020.1730392. Epub 2020 Feb 20.
Coat color is one of the major quality traits of animals, and miR-129-5p acts as an important regulator for melanin biosynthesis in mammals. In this study, real-time PCR and western blotting were used to examine the expression of miR-129-5p and its targets genes in the skin of different coat color goats. The results showed that the expression of miR-129-5p in the skin samples of Inner Mongolia cashmere goats (IMCG) was higher than that of Dazu black goat (DBG). Also, the target genes (tyrosinase (), frizzled 6 () and glycogen synthase kinase 3β ()) of miR-129-5p was highly expressed in the skin samples of DBG. The expression of miR-129-5p firstly increased and then decreased with age in F1 hybrid generation of DBG and IMCG. In addition, the expression of decreased with age, while the expression of increased with age but then decreased. The expression of and in the skin samples of F1 of different ages were irregular. Our results indicated that miR-129-5p mainly affects the formation of coat color of goats by decreasing the expression of . This study suggests that miR-129-5p can act as a suppressor in the formation of coat color to lay the foundation for studying the effect of miR-129-5p on melanin synthesis.
毛色是动物的主要质量性状之一，miR-129-5p是哺乳动物黑色素生物合成的重要调节因子。本研究采用实时荧光定量PCR和蛋白质免疫印迹法检测不同毛色山羊皮肤中miR-129-5p及其靶基因的表达。结果表明，内蒙古绒山羊（IMCG）皮肤样本中miR-129-5p的表达高于大足黑山羊（DBG）。此外，miR-129-5p的靶基因（酪氨酸酶（）、卷曲蛋白6（）和糖原合酶激酶3β（））在DBG皮肤样本中高表达。在DBG和IMCG的F1杂交后代中，miR-129-5p的表达随年龄增长先升高后降低。此外，的表达随年龄增长而降低，而的表达随年龄增长先升高后降低。不同年龄F1皮肤样本中的和表达无规律。我们的结果表明，miR-129-5p主要通过降低的表达来影响山羊毛色的形成。本研究表明，miR-129-5p在毛色形成中可作为一种抑制因子，为研究miR-129-5p对黑色素合成的影响奠定基础。
