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鉴定和分析与绒山羊皮肤组织中羊绒纤维特性相关的 miRNAs。

The Identification and Characteristics of miRNAs Related to Cashmere Fiber Traits in Skin Tissue of Cashmere Goats.

机构信息

Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

Genes (Basel). 2023 Feb 12;14(2):473. doi: 10.3390/genes14020473.

DOI:10.3390/genes14020473
PMID:36833400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9957446/
Abstract

microRNAs (miRNAs) are involved in the regulation of biological phenomena by down-regulating the expression of mRNAs. In this study, Liaoning cashmere (LC) goats ( = 6) and Ziwuling black (ZB) goats ( = 6) with different cashmere fiber production performances were selected. We supposed that miRNAs are responsible for the cashmere fiber trait differences. To test the hypothesis, the expression profiles of miRNAs from the skin tissue of the two caprine breeds were compared using small RNA sequencing (RNA-seq). A total of 1293 miRNAs were expressed in the caprine skin samples, including 399 known caprine miRNAs, 691 known species-conserved miRNAs, and 203 novel miRNAs. Compared with ZB goats, 112 up-regulated miRNAs, and 32 down-regulated miRNAs were found in LC goats. The target genes of the differentially expressed miRNAs were remarkably concentrated on some terms and pathways associated with cashmere fiber performance, including binding, cell, cellular protein modification process, and Wnt, Notch, and MAPK signaling pathways. The miRNA-mRNA interaction network found that 14 miRNAs selected may contribute to cashmere fiber traits regulation by targeting functional genes associated with hair follicle activities. The results have reinforced others leading to a solid foundation for further investigation of the influences of individual miRNAs on cashmere fiber traits in cashmere goats.

摘要

微小 RNA（miRNA）通过下调 mRNA 的表达来参与调控生物现象。在这项研究中，选择了具有不同羊绒纤维生产性能的辽宁绒山羊（LC）（=6）和籽乌陵黑山羊（ZB）（=6）。我们假设 miRNA 是导致羊绒纤维性状差异的原因。为了验证这一假设，我们使用小 RNA 测序（RNA-seq）比较了两种山羊品种皮肤组织中的 miRNA 表达谱。在山羊皮肤样本中总共表达了 1293 个 miRNA，包括 399 个已知的山羊 miRNA、691 个已知物种保守的 miRNA 和 203 个新的 miRNA。与 ZB 山羊相比，LC 山羊中有 112 个上调的 miRNA 和 32 个下调的 miRNA。差异表达 miRNA 的靶基因显著集中在一些与羊绒纤维性能相关的术语和途径上，包括结合、细胞、细胞蛋白修饰过程以及 Wnt、Notch 和 MAPK 信号通路。发现的 miRNA-mRNA 相互作用网络表明，14 个选定的 miRNA 可能通过靶向与毛囊活动相关的功能基因来调节羊绒纤维性状。这些结果为进一步研究个别 miRNA 对绒山羊羊绒纤维性状的影响提供了坚实的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/6930033e2ae6/genes-14-00473-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/0be4963995b0/genes-14-00473-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/f383eeb6abb5/genes-14-00473-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/0a0a36cb76af/genes-14-00473-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/7b97bdd76314/genes-14-00473-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/7b375c484474/genes-14-00473-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/6930033e2ae6/genes-14-00473-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/0be4963995b0/genes-14-00473-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/f383eeb6abb5/genes-14-00473-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/0a0a36cb76af/genes-14-00473-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/7b97bdd76314/genes-14-00473-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/7b375c484474/genes-14-00473-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6e6/9957446/6930033e2ae6/genes-14-00473-g006.jpg

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本文引用的文献

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