Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
Int J Environ Res Public Health. 2020 Feb 16;17(4):1266. doi: 10.3390/ijerph17041266.
The water-level fluctuation zone (WLFZ) is a transitional zone between terrestrial and aquatic ecosystems. Plant communities that are constructed artificially in the WLFZ can absorb and retain nutrients such as nitrogen (N) and phosphorus (P). However, the microbial community composition and function associated with this process have not been elucidated. In this study, four artificially constructed plant communities, including those of herbs ( and ), trees (), and shrubs () from the newly formed WLFZ of the Danjiangkou Reservoir were evaluated. The bacterial community compositions were analyzed by 16S rRNA gene sequencing using a MiSeq platform, and the functions of these communities were assessed via Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis. The results showed that the bacterial communities primarily comprised 362 genera from 24 phyla, such as Proteobacteria, Acidobacteria, Actinobacteria, and Gemmatimonadetes, showing the richness of the community composition. Planting altered the bacterial community composition, with varying effects observed among the different plant types. The bacterial community functional analysis revealed that these bacteria were primarily associated with six biological metabolic pathway categories (e.g., metabolism, genetic information processing, and environmental information processing) with 34 subfunctions, showing the richness of community functions. The planting of , , and improved the metabolic capabilities of bacterial communities. N- and P-cycling gene analysis showed that planting altered the N- and P-cycling metabolic capacities of soil bacteria. The overall N- and P-metabolic capacity was highly similar between and samples and between and samples. The results of this study provide a preliminary analysis of soil bacterial community structure and function in the WLFZ of the Danjiangkou Reservoir and provides a reference for vegetation construction in this zone.
水位波动带（WLFZ）是陆地和水生生态系统之间的过渡带。在 WLFZ 中人工构建的植物群落可以吸收和保留氮（N）和磷（P）等营养物质。然而，与该过程相关的微生物群落组成和功能尚未阐明。在这项研究中，评估了丹江口水库新形成的 WLFZ 的四种人工构建的植物群落，包括草本植物（和）、乔木（）、和灌木（）。通过 MiSeq 平台对 16S rRNA 基因测序分析了细菌群落组成，并通过 Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) 分析评估了这些群落的功能。结果表明，细菌群落主要由 24 个门的 362 个属组成，如 Proteobacteria、Acidobacteria、Actinobacteria 和 Gemmatimonadetes，显示出群落组成的丰富度。种植改变了细菌群落组成，不同植物类型的影响不同。细菌群落功能分析表明，这些细菌主要与六个生物代谢途径类别（如代谢、遗传信息处理和环境信息处理）相关联，具有 34 个子功能，显示出群落功能的丰富度。种植、和可以提高细菌群落的代谢能力。N 和 P 循环基因分析表明，种植改变了土壤细菌的 N 和 P 循环代谢能力。和样本以及和样本之间的整体 N 和 P 代谢能力非常相似。本研究结果初步分析了丹江口水库 WLFZ 土壤细菌群落结构和功能，为该区域的植被建设提供了参考。
