Institute of Cardiovascular Sciences, College of Medical and Dental Science, University of Birmingham , Birmingham, UK.
Centre of Membrane Proteins and Receptors, Universities of Birmingham and Nottingham , UK.
Platelets. 2020 Jul 3;31(5):559-569. doi: 10.1080/09537104.2020.1732321. Epub 2020 Feb 21.
Recent advances in super-resolution (sub-diffraction limited) microscopy have yielded remarkable insights into the nanoscale architecture and behavior of cells. In addition to the capacity to provide sub 100 nm resolution, these technologies offer unique quantitative opportunities with particular relevance to platelet and megakaryocyte biology. In this review, we provide a short introduction to modern super-resolution microscopy, its applications in the field of platelet and megakaryocyte biology, and emerging quantitative approaches which will allow for unprecedented insights into the biology of these unique cell types.
近年来，超分辨率（亚衍射极限）显微镜技术的发展取得了显著的进展，使人们对细胞的纳米级结构和行为有了更深入的了解。除了能够提供小于 100nm 的分辨率外，这些技术还提供了独特的定量机会，特别是与血小板和巨核细胞生物学相关。在这篇综述中，我们将对现代超分辨率显微镜技术进行简要介绍，讨论其在血小板和巨核细胞生物学领域的应用，以及新兴的定量方法，这些方法将使人们对这些独特细胞类型的生物学有前所未有的深入了解。
