Androsov V V, Vysotskiĭ V V
Zh Mikrobiol Epidemiol Immunobiol. 1977(1):94-9.
The author studied the ultrastructure of two spherical E. coli K12 mutants (llr) obtained under the effect of N-nitroso-N-methylurea. Seven morphological types of cells differing from one another by shape, size and cytoarchitectonics were distinguished. Superficial structures of the majority of the cells were represented by the membranes of the cell wall and the cytoplasmic membrane of common structure. Some of the cells had only one membrane coat and a high electron optic density of the cytoplasm. Transitional forms of cells were also encountered. The ultrastructure of each morphological type in the population of the llr-mutants was described in detail. The capacity of the mutants to vacuolization, to the intra- and extracellular budding, and also the ability to form multiple membrane structures resembled analogous structures of stable L-forms of the Gram-negative microbes. The problems of morphological differentiation of the L-forms and of the llr-mutants, and also problems connected with the formation of the multiple membrane structures and small elemental bodies in the cells of the llr-mutants are discussed.
作者研究了在N-亚硝基-N-甲基脲作用下获得的两个球形大肠杆菌K12突变体（llr）的超微结构。区分出了七种在形状、大小和细胞结构上彼此不同的细胞形态类型。大多数细胞的表面结构由具有共同结构的细胞壁膜和细胞质膜组成。一些细胞只有一层膜包被且细胞质具有高电子光学密度。还遇到了细胞的过渡形式。详细描述了llr突变体群体中每种形态类型的超微结构。突变体的空泡化能力、细胞内和细胞外出芽能力以及形成多个膜结构的能力类似于革兰氏阴性微生物稳定L型的类似结构。讨论了L型和llr突变体的形态分化问题，以及与llr突变体细胞中多个膜结构和小的原生小体形成相关的问题。
