Budtz-Jorgensen E
Acta Allergol. 1977 Feb;32(1):15-26.
Leukocyte migration inhibition by antigen from Candida albicans and Fusobacterium nucleatum was studied in man by means of the direct leukocyte migration agarose technique (LMAT). Antigens were prepared by prolonged ultrasonication and were added to the leukocyte cultures in the original as well as in the concentrated solutions. Significant inhibition of migration with a 10-fold concentrated solution of candida antigen was demonstrated in subjects showing apositive intracutaneous reaction to candida. With the fusobacterial antigen, inhibition of migration or stimulation of migration were demonstrated in subjects with slight peridontal inflammation. A 5-fold increase in the concentration of fusobacterial antigen resulted in increased stimulation of migration or inhibition of migration. Preincubation of the leukocytes with puromycin abolished both inhibition and stimulation of migration by candidal or fusobacterial antigen. The study confirms that it is important to study dose-related effects when applying the LMAT to test cell-mediated immunity. The study is in support of stimulation of migration being an immunologic reaction.
采用直接白细胞迁移琼脂糖技术（LMAT）在人体中研究了白色念珠菌和具核梭杆菌抗原对白细胞迁移的抑制作用。抗原通过长时间超声处理制备，并以原液和浓缩液形式添加到白细胞培养物中。在对念珠菌皮内反应呈阳性的受试者中，使用10倍浓缩的念珠菌抗原溶液可显著抑制迁移。对于具核梭杆菌抗原，在患有轻度牙周炎的受试者中观察到迁移抑制或迁移刺激。具核梭杆菌抗原浓度增加5倍会导致迁移刺激增加或迁移抑制。用嘌呤霉素预孵育白细胞可消除念珠菌或具核梭杆菌抗原对迁移的抑制和刺激作用。该研究证实，在应用LMAT检测细胞介导免疫时，研究剂量相关效应很重要。该研究支持迁移刺激是一种免疫反应。
