Four new pregnane glycosides from and their -glucosidase and -amylase inhibitory activities.

Four new pregnane glycosides, gymlatifosides A - D () and one known pregnane glycoside, verticilloside J () were isolated from the leaves of Wall. ex Wight. Their chemical structures were elucidated on the basis of extensive spectroscopic methods, including 1D, 2D NMR, HR-ESI-MS, and in comparison with the reported data. All these compounds were tested for -glucosidase and -amylase inhibitory activities. Compound exhibited the most anti -glucosidase activity with inhibitory percentage of 37.8 ± 1.5% at the concentration of 200 μM. Compounds showed moderate anti -glucosidase activity with inhibitory percentage ranging from 7.0 to 30.1%. In addition, all compounds showed moderate/weak anti -amylase activity in the investigated test.