冷冻保存后培养的人神经元细胞活力得到证实。

Human neuronal cell viability demonstrated in culture after cryopreservation.

作者信息

Silani V, Pizzuti A, Strada O, Falini A, Buscaglia M, Scarlato G

机构信息

Dino Ferrari Center, University of Milan Medical School, Italy.

出版信息

Brain Res. 1988 Nov 8;473(1):169-74. doi: 10.1016/0006-8993(88)90331-9.

DOI:10.1016/0006-8993(88)90331-9

PMID:3208121

Abstract

A human neuronal cell freezing technique has been developed. The results indicate that human fetal neuronal cells can be frozen with 7%-10% dimethyl sulfoxide (DMSO) as cryoprotectant. The survival of isolated thawed cells has been evaluated in culture. Cells have been frozen at -196 degrees C for 370 days without loss of viability. Average recovery rate of frozen cells was 62% of the recovery rate of cultured unfrozen controls. Thawed cells show neurite outgrowth and maintain both cellular markers such as neuron specific enolase (NSE) and neurochemical characteristics (GABA synthesis). Morphological integrity of cryopreserved neurons has been confirmed at ultrastructural level.

摘要

一种人类神经元细胞冷冻技术已被开发出来。结果表明，人类胎儿神经元细胞可用7%-10%的二甲基亚砜（DMSO）作为冷冻保护剂进行冷冻。已在培养中评估了分离后解冻细胞的存活率。细胞已在-196℃下冷冻370天而无活力丧失。冷冻细胞的平均恢复率为未冷冻培养对照恢复率的62%。解冻后的细胞表现出神经突生长，并维持神经元特异性烯醇化酶（NSE）等细胞标志物以及神经化学特性（GABA合成）。已在超微结构水平证实了冷冻保存神经元的形态完整性。