人类卵母细胞的冷冻保存

Cryopreservation of human oocytes.

作者信息

Al-Hasani S, Diedrich K, van der Ven H, Reinecke A, Hartje M, Krebs D

机构信息

Universitäts-Frauenklinik Bonn, Bonn-Venusberg, FRG.

出版信息

Hum Reprod. 1987 Nov;2(8):695-700. doi: 10.1093/oxfordjournals.humrep.a136616.

DOI:10.1093/oxfordjournals.humrep.a136616

PMID:3437048

Abstract

Three different methods were used for freezing human excess oocytes (320 frozen, 205 thawed) in our IVF programme and the results of these methods were compared. A high fertilization rate (75%) could be achieved after thawing, using 1,2 propanediol as a cryoprotectant. Polyploidy rates of 20% and 40% were observed using DMSO and 1,2-propanediol as cryoprotectants, respectively. Using the ultracooling method, the survival rate was poor (4%).

摘要

在我们的体外受精项目中，使用了三种不同的方法冷冻人类多余的卵母细胞（冷冻320个，解冻205个），并比较了这些方法的结果。使用1,2 - 丙二醇作为冷冻保护剂解冻后可实现较高的受精率（75%）。分别使用二甲基亚砜（DMSO）和1,2 - 丙二醇作为冷冻保护剂时，观察到多倍体率分别为20%和40%。使用超低温冷却方法时，存活率较低（4%）。