Sperling S
Acta Ophthalmol (Copenh). 1977 Feb;55(1):1-8. doi: 10.1111/j.1755-3768.1977.tb06090.x.
The construction and operation of an apparatus for controlled rate corneal freezing is described. The cooling is obtained by close thermal contact between a vial holding the cornea and a metal core. A predetermined cooling rate is achieved by partial immersion of the core in a fixed amount of liquid nitrogen. In a preliminary series greater than or equal to 95% endothelial survival was obtained.
本文描述了一种用于控制角膜冷冻速率的仪器的构建与操作。冷却通过装有角膜的小瓶与金属芯之间的紧密热接触来实现。通过将金属芯部分浸入固定量的液氮中可达到预定的冷却速率。在初步系列实验中，获得了大于或等于95%的内皮细胞存活率。
