Ruusuvaara P, Setälä K
Department of Ophthalmology, University of Helsinki, Finland.
Acta Ophthalmol (Copenh). 1988 Dec;66(6):687-91. doi: 10.1111/j.1755-3768.1988.tb04062.x.
This paper deals with the long-term survival of corneal transplant endothelial cells. Endothelia of 17 cryopreserved corneal transplants were photographed with a specular microscope, first 1.5 years (mean) following transplantation, and again 10 years later. All transplants were absolutely clear. The mean cell loss was 23% in 12 years. After 2 to 14 years post-operatively the cell loss was noticed to be only 13.6% (from 990 +/- 255 cells/mm2 to 855 +/- 213 cells/mm2). It seems that endothelial cells of cryopreserved corneal grafts, although few in number, are still quite resistant and viable after a long follow-up time and survive like endothelial cells after preservation in tissue culture media.
本文探讨了角膜移植内皮细胞的长期存活情况。用镜面显微镜对17例冷冻保存的角膜移植片的内皮进行拍照，第一次是在移植后平均1.5年，10年后再次拍照。所有移植片均完全透明。12年中平均细胞丢失率为23%。术后2至14年，细胞丢失率仅为13.6%（从990±255个细胞/mm²降至855±213个细胞/mm²）。冷冻保存的角膜移植物的内皮细胞似乎虽然数量较少，但经过长时间随访后仍具有相当的抵抗力和活力，并且像在组织培养基中保存后的内皮细胞一样存活。
