State Key Laboratory of Oral Diseases and National Clinical Research Centre for Oral Diseases, Sichuan University, No.14, Section 3 of Renmin Road South, Chengdu, 610041, China; Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, No.14, Section 3 of Renmin Road South, Chengdu, 610041, China.
State Key Laboratory of Oral Diseases and National Clinical Research Centre for Oral Diseases, Sichuan University, No.14, Section 3 of Renmin Road South, Chengdu, 610041, China; Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, No.14, Section 3 of Renmin Road South, Chengdu, 610041, China.
J Dent. 2020 Mar;94:103301. doi: 10.1016/j.jdent.2020.103301. Epub 2020 Feb 18.
The aim of this study was to analyse the protein profiles of acquired enamel pellicle (AEP) at two time points (5 min and 2 h) between caries-susceptible and caries-free subjects using label-free and parallel reaction monitoring (PRM) proteomic approaches, and to discover potential biomarkers for dental caries.
Sixty participants (30 caries-susceptible (DMFT>5) and 30 caries-free subjects (DMFT = 0)) were included. Their AEP at 5 min and 2 h was separately sampled, and the AEP materials were quantitatively analysed using label-free proteomics. Bioinformatics analysis of differentially expressed proteins was subsequently conducted and target proteins were verified using PRM analysis.
A total of 82 and 39 differentially expressed proteins were identified at the 5-minute and 2 -h points, respectively, between the caries-susceptible and caries-free groups. Some proteins related to immune response and antibacterial activity were identified, and the characteristic enriched biological processes of up- and down-regulated proteins were presented using bioinformatics analysis. Subsequently, five intriguing target proteins of each group were chosen for PRM analysis and their presence was successfully verified in the 5-minute and 2 -h AEPs, respectively.
The proteins in the AEPs of caries-susceptible and caries-free subjects at both time points presented unique protein profiles related to immune response and antibacterial activity. Mucin-7 can be regarded as an important potential AEP biomarker for dental caries.
The results of the study identified potential biomarkers, which can facilitate the design of new bio-functional agents for the diagnosis and treatment of dental caries in future.
本研究旨在使用无标记和平行反应监测（PRM）蛋白质组学方法，分析在龋齿易感和无龋受试者之间两个时间点（5 分钟和 2 小时）获得的牙釉质获得性牙菌斑（AEP）的蛋白质谱，并发现潜在的龋齿生物标志物。
纳入 60 名受试者（30 名龋齿易感（DMFT>5）和 30 名无龋受试者（DMFT=0））。分别采集他们在 5 分钟和 2 小时的 AEP，并使用无标记蛋白质组学对 AEP 材料进行定量分析。随后对差异表达蛋白进行生物信息学分析，并使用 PRM 分析验证靶蛋白。
在龋齿易感和无龋组之间，分别在 5 分钟和 2 小时点鉴定出 82 和 39 个差异表达蛋白。鉴定出一些与免疫反应和抗菌活性相关的蛋白质，并使用生物信息学分析呈现了上调和下调蛋白的特征丰富的生物学过程。随后，选择每个组的五个有趣的靶蛋白进行 PRM 分析，并分别成功验证了它们在 5 分钟和 2 小时 AEP 中的存在。
在两个时间点，龋齿易感和无龋受试者的 AEP 中的蛋白质呈现出与免疫反应和抗菌活性相关的独特蛋白质谱。粘蛋白-7 可被视为一种重要的潜在牙釉质获得性牙菌斑生物标志物。
该研究的结果确定了潜在的生物标志物，这有助于未来为龋齿的诊断和治疗设计新的生物功能制剂。
