Abed Raeid M M, Shanti Mary, Muthukrishnan Thirumahal, Al-Riyami Zayana, Pracejus Bernhard, Moraetis Daniel
Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman.
Earth Science Department, College of Science, Sultan Qaboos University, Muscat, Oman.
Front Microbiol. 2020 Jan 29;11:12. doi: 10.3389/fmicb.2020.00012. eCollection 2020.
Microbial mats are rarely reported for chromium-polluted ecosystems, hence information on their bacterial diversity and role in chromium removal are very scarce. We investigated the role of nine microbial mats, collected from three quarry sumps of chromium mining sites, in the removal of hexavalent chromium [Cr(VI)]. Bacterial diversity in these mats and community shifts after incubation with Cr(VI) have been investigated using MiSeq sequencing. In nature, a chromium content of 1,911 ± 100 mg kg was measured in the microbial mats, constituting the third highest source of environmentally available chromium. The mats were able to remove 1 mg l of Cr(VI) in 7 days under aerobic conditions. MiSeq sequencing of the original mats yielded 46-99% of the sequences affiliated to Proteobacteria, Firmicutes and Actinobacteria. When the mats were incubated with Cr(VI), the bacterial community shifted in the favor of Alphaproteobacteria and Verrucomicrobiae. We conclude that microbial mats in the quarry sumps harbor diverse microorganisms with the ability to remove toxic Cr(VI), hence these mats can be potentially used to remove chromium from polluted waters.
关于铬污染生态系统中微生物席的报道很少，因此关于其细菌多样性以及在铬去除中作用的信息非常匮乏。我们研究了从铬矿开采场地的三个采石场积水坑采集的九个微生物席在去除六价铬[Cr(VI)]方面的作用。使用MiSeq测序技术研究了这些微生物席中的细菌多样性以及与Cr(VI)孵育后的群落变化。在自然环境中，微生物席中的铬含量测定为1911±100毫克/千克，是环境中可利用铬的第三大来源。这些微生物席在有氧条件下能够在7天内去除1毫克/升的Cr(VI)。对原始微生物席进行MiSeq测序，结果显示46% - 99%的序列隶属于变形菌门、厚壁菌门和放线菌门。当微生物席与Cr(VI)孵育时，细菌群落向有利于α-变形菌纲和疣微菌门的方向转变。我们得出结论，采石场积水坑中的微生物席含有多种具有去除有毒Cr(VI)能力的微生物，因此这些微生物席有可能用于从污染水体中去除铬。
