School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Department of Orthopedics, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Int J Biol Macromol. 2020 Jun 15;153:1-16. doi: 10.1016/j.ijbiomac.2020.02.173. Epub 2020 Feb 18.
Over the past decade, various composite materials fabricated using natural or synthetic biopolymers incorporated with bioceramic have been widely investigated for the regeneration of segmental bone defect. In the present study, nano-bioglass incorporated osteoconductive composite scaffolds were fabricated through polyelectrolyte complexation/phase separation and resuspension of separated complex in gelatin matrix. Developed scaffold exhibits controlled bioreactivity, minimize abrupt pH rise (~7.8), optimal swelling behavior (2.6+-3.1) and enhances mechanical strength (0.62 ± 0.18 MPa) under wet condition. Moreover, in-vitro cell study shows that the fabricated scaffold provide suitable template for cellular attachment, spreading, biomineralization and collagen based matrix deposition. Also, the developed scaffold was evaluated for biocompatibility and bone tissue regeneration potential through implantation in non-union segmental bone defect created in rabbit animal model. The obtained histological analysis indicates strong potential of the composite scaffold for bone tissue regeneration, vascularization and reconstruction of defects. Thus, the developed composite scaffold might be a suitable biomaterial for bone tissue engineering applications.
在过去的十年中，广泛研究了使用天然或合成生物聚合物与生物陶瓷复合制成的各种复合材料，以用于节段性骨缺损的再生。在本研究中，通过聚电解质络合/相分离以及将分离的复合物重新悬浮在明胶基质中来制备掺入纳米生物玻璃的骨传导性复合支架。所开发的支架具有可控制的生物反应性、最小化急剧的 pH 值升高（约 7.8）、最佳的溶胀行为（2.6+-3.1）和在湿条件下增强的机械强度（0.62±0.18 MPa）。此外，体外细胞研究表明，所制备的支架为细胞附着、扩散、生物矿化和基于胶原蛋白的基质沉积提供了合适的模板。还通过在兔动物模型中创建的非愈合性节段性骨缺损中植入来评估所开发的支架的生物相容性和骨组织再生潜力。获得的组织学分析表明，该复合材料支架具有很强的骨组织再生、血管生成和缺陷重建的潜力。因此，所开发的复合支架可能是骨组织工程应用的合适生物材料。
