Ferroni Letizia, Zago Michela, Patergnani Simone, Campbell Shannon E, Hébert Lise, Nielsen Michael, Scarpa Carlotta, Bassetto Franco, Pinton Paolo, Zavan Barbara
Maria Cecilia Hospital, GVM Care & Research, 48033 Cotignola (RA), Italy.
Department of Medical Sciences, University of Ferrara, 44121 Ferrara, Italy.
J Clin Med. 2020 Feb 18;9(2):559. doi: 10.3390/jcm9020559.
Fluorescent light energy (FLE) has been used to treat various injured tissues in a non-pharmacological and non-thermal fashion. It was applied to stimulate cell proliferation, accelerate healing in chronic and acute wounds, and reduce pain and inflammation. FLE has been shown to reduce pro-inflammatory cytokines while promoting an environment conducive to healing. A possible mechanism of action of FLE is linked to regulation of mitochondrial homeostasis. This work aims to investigate the effect of FLE on mitochondrial homeostasis in an in vitro model of inflammation. Confocal microscopy and gene expression profiling were performed on cultures of inflamed human dermal fibroblasts treated with either direct light from a multi-LED lamp, or FLE from either an amorphous gel or sheet hydrogel matrix. Assessment using confocal microscopy revealed mitochondrial fragmentation in inflamed cells, likely due to exposure to inflammatory cytokines, however, mitochondrial networks were restored to normal 24-h after treatment with FLE. Moreover, gene expression analysis found that treatment with FLE resulted in upregulation of uncoupling protein 1 () and carnitine palmitoyltransferase 1B () genes, which encode proteins favoring mitochondrial ATP production through oxidative phosphorylation and lipid β-oxidation, respectively. These observations demonstrate a beneficial effect of FLE on mitochondrial homeostasis in inflamed cells.
荧光光能（FLE）已被用于以非药物和非热的方式治疗各种受损组织。它被应用于刺激细胞增殖、加速慢性和急性伤口的愈合，并减轻疼痛和炎症。FLE已被证明可减少促炎细胞因子，同时促进有利于愈合的环境。FLE的一种可能作用机制与线粒体稳态的调节有关。这项工作旨在研究FLE在炎症体外模型中对线粒体稳态的影响。对用多LED灯的直射光、无定形凝胶或片状水凝胶基质的FLE处理的炎症人皮肤成纤维细胞培养物进行共聚焦显微镜检查和基因表达谱分析。使用共聚焦显微镜进行的评估显示，炎症细胞中存在线粒体碎片化，这可能是由于暴露于炎性细胞因子所致，然而，在用FLE处理24小时后，线粒体网络恢复正常。此外，基因表达分析发现，用FLE处理导致解偶联蛋白1（）和肉碱棕榈酰转移酶1B（）基因上调，这两种基因分别编码有利于通过氧化磷酸化和脂质β氧化产生线粒体ATP的蛋白质。这些观察结果证明了FLE对炎症细胞中线粒体稳态的有益作用。
