Department of Biology, Biological Science Center, Universidade Estadual do Norte do Paraná - UENP, Luiz Meneghel Campus, Bandeirantes, 8630-000 Paraná, Brazil.
Department of Biochemistry, Universidade Estadual de Maringá - UEM, Maringá, 87020-900 Paraná, Brazil.
Biol Open. 2020 Feb 28;9(3):bio047282. doi: 10.1242/bio.047282.
As the number of overweight and obese people has risen in recent years, there has been a parallel increase in the number of people with metabolic syndrome, diabetes and non-alcoholic fatty liver disease. The consumption of artificially sweetened beverages contributes to these epidemics. This study investigated the long-term effects of ingestion of a 40% sucrose solution on serum and hepatic parameters in male Wistar rats (). After 180 days, the glycemic response, lipid profile and hepatic oxidative stress were compared to those of rats maintained on water. Sucrose ingestion led to higher body weight, increased fat deposits, reduced voluntary food intake and reduced feeding efficiency. Rats that received sucrose solution showed early signs of glucose intolerance and insulin resistance, such as hyperinsulinemia. Serum triacylglycerol (TG), very-low density lipoprotein (VLDL), cholesterol, ALT and AST levels increased after sucrose consumption. Elevated malondialdehyde and superoxide dismutase (SOD) levels and reduced glutathione levels characterize the hepatic oxidative stress due to sucrose ingestion. Liver sample histology showed vacuolar traces and increased fibrotic tissue. Our data showed the harmful effects of chronic consumption of sucrose solution, which can cause alterations that are found frequently in obesity, glucose intolerance and non-alcoholic hepatic disease, characteristics of metabolic syndrome.
近年来，随着超重和肥胖人群的增加，代谢综合征、糖尿病和非酒精性脂肪肝患者的数量也呈平行增加。人工甜味饮料的消费导致了这些流行疾病的发生。本研究旨在调查雄性 Wistar 大鼠长期摄入 40%蔗糖溶液对血清和肝脏参数的影响（）。180 天后，将其血糖反应、血脂谱和肝脏氧化应激与维持用水的大鼠进行比较。蔗糖摄入导致体重增加、脂肪堆积、自愿食物摄入量减少和进食效率降低。摄入蔗糖溶液的大鼠表现出葡萄糖不耐受和胰岛素抵抗的早期迹象，如高胰岛素血症。血清三酰甘油（TG）、极低密度脂蛋白（VLDL）、胆固醇、ALT 和 AST 水平在蔗糖消耗后升高。蔗糖摄入导致肝氧化应激的特征是丙二醛和超氧化物歧化酶（SOD）水平升高，谷胱甘肽水平降低。肝组织学样本显示空泡痕迹和纤维化组织增加。我们的数据显示了慢性摄入蔗糖溶液的有害影响，这可能导致肥胖、葡萄糖不耐受和非酒精性肝病中经常发现的改变，这些改变是代谢综合征的特征。
