邮政编码结合蛋白1（ZBP1；IGF2BP1）：序列特异性RNA调控模型
Zipcode Binding Protein 1 (ZBP1; IGF2BP1): A Model for Sequence-Specific RNA Regulation.
作者信息
Biswas Jeetayu, Nunez Leti, Das Sulagna, Yoon Young J, Eliscovich Carolina, Singer Robert H
机构信息
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
出版信息
Cold Spring Harb Symp Quant Biol. 2019;84:1-10. doi: 10.1101/sqb.2019.84.039396. Epub 2020 Feb 21.
Abstract
The fate of an RNA, from its localization, translation, and ultimate decay, is dictated by interactions with RNA binding proteins (RBPs). β-actin mRNA has functioned as the classic example of RNA localization in eukaryotic cells. Studies of β-actin mRNA over the past three decades have allowed understanding of how RBPs, such as ZBP1 (IGF2BP1), can control both RNA localization and translational status. Here, we summarize studies of β-actin mRNA and focus on how ZBP1 serves as a model for understanding interactions between RNA and their binding protein(s). Central to the study of RNA and RBPs were technological developments that occurred along the way. We conclude with a future outlook highlighting new technologies that may be used to address still unanswered questions about RBP-mediated regulation of mRNA during its life cycle, within the cell.
摘要
RNA的命运，从其定位、翻译到最终降解，都由与RNA结合蛋白（RBP）的相互作用所决定。β-肌动蛋白mRNA一直是真核细胞中RNA定位的经典例子。在过去三十年里，对β-肌动蛋白mRNA的研究使人们了解了诸如ZBP1（IGF2BP1）等RBP如何控制RNA的定位和翻译状态。在这里，我们总结了对β-肌动蛋白mRNA的研究，并重点关注ZBP1如何作为理解RNA与其结合蛋白之间相互作用的模型。RNA和RBP研究的核心是在此过程中出现的技术发展。我们最后展望未来，强调可能用于解决关于细胞内RBP介导的mRNA生命周期调控中仍未解答问题的新技术。
相似文献
1
Zipcode Binding Protein 1 (ZBP1; IGF2BP1): A Model for Sequence-Specific RNA Regulation.邮政编码结合蛋白1（ZBP1；IGF2BP1）：序列特异性RNA调控模型
Cold Spring Harb Symp Quant Biol. 2019;84:1-10. doi: 10.1101/sqb.2019.84.039396. Epub 2020 Feb 21.
2
Different motif requirements for the localization zipcode element of β-actin mRNA binding by HuD and ZBP1.HuD和ZBP1与β-肌动蛋白mRNA结合的定位邮政编码元件的不同基序要求。
Nucleic Acids Res. 2015 Sep 3;43(15):7432-46. doi: 10.1093/nar/gkv699. Epub 2015 Jul 7.
3
Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.IGF2BP1[Formula: see text] 小鼠中标记肌动蛋白 mRNA 失调。
Proc Natl Acad Sci U S A. 2022 Sep 13;119(37):e2208465119. doi: 10.1073/pnas.2208465119. Epub 2022 Sep 6.
4
Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1.通过Src依赖的ZBP1磷酸化对β-肌动蛋白翻译进行空间调控。
Nature. 2005 Nov 24;438(7067):512-5. doi: 10.1038/nature04115.
5
Localization of a beta-actin messenger ribonucleoprotein complex with zipcode-binding protein modulates the density of dendritic filopodia and filopodial synapses.一种β-肌动蛋白信使核糖核蛋白复合体与邮政编码结合蛋白的定位调节树突丝状伪足和丝状伪足突触的密度。
J Neurosci. 2003 Nov 12;23(32):10433-44. doi: 10.1523/JNEUROSCI.23-32-10433.2003.
6
ZBP1 recognition of beta-actin zipcode induces RNA looping.ZBP1 通过识别β-肌动蛋白的 zipcode 诱导 RNA 环化。
Genes Dev. 2010 Jan 15;24(2):148-58. doi: 10.1101/gad.1862910.
7
Two ZBP1 KH domains facilitate beta-actin mRNA localization, granule formation, and cytoskeletal attachment.两个ZBP1 KH结构域促进β-肌动蛋白mRNA的定位、颗粒形成和细胞骨架附着。
J Cell Biol. 2003 Jan 6;160(1):77-87. doi: 10.1083/jcb.200206003. Epub 2002 Dec 30.
8
Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization.转录和定位过程中ZBP1与β-肌动蛋白mRNA结合的实时可视化。
Curr Biol. 2003 Feb 4;13(3):199-207. doi: 10.1016/s0960-9822(03)00044-7.
9
Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility.KIF11与ZBP1的特异性相互作用调节β-肌动蛋白mRNA的运输和细胞运动。
J Cell Sci. 2015 Mar 1;128(5):1001-10. doi: 10.1242/jcs.161679. Epub 2015 Jan 14.
10
Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control.RNA 邮政编码的空间排列确定了受转录后控制的 mRNAs。
Genes Dev. 2012 Jan 1;26(1):43-53. doi: 10.1101/gad.177428.111.
引用本文的文献
1
Axonal RNA localization is essential for long-term memory.轴突RNA定位对长期记忆至关重要。
Nat Commun. 2025 Mar 15;16(1):2560. doi: 10.1038/s41467-025-57651-7.
2
Construction destruction: Contribution of dyregulated proteostasis to neurodevelopmental disorders.结构破坏：蛋白稳态失调对神经发育障碍的影响
Curr Opin Neurobiol. 2025 Feb;90:102934. doi: 10.1016/j.conb.2024.102934. Epub 2024 Nov 28.
3
An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.一项基于图像的RNA干扰筛选确定表皮生长因子受体（EGFR）信号通路是IMP核糖核蛋白颗粒的调节因子。
J Cell Sci. 2024 Dec 1;137(23). doi: 10.1242/jcs.262119. Epub 2024 Dec 10.
4
RNA-Binding Proteins: A Role in Neurotoxicity?RNA 结合蛋白：在神经毒性中起作用？
Neurotox Res. 2023 Dec;41(6):681-697. doi: 10.1007/s12640-023-00669-w. Epub 2023 Sep 30.
5
Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.IGF2BP1[Formula: see text] 小鼠中标记肌动蛋白 mRNA 失调。
Proc Natl Acad Sci U S A. 2022 Sep 13;119(37):e2208465119. doi: 10.1073/pnas.2208465119. Epub 2022 Sep 6.
6
Emerging Roles of RNA-Binding Proteins in Neurodevelopment.RNA结合蛋白在神经发育中的新作用
J Dev Biol. 2022 Jun 10;10(2):23. doi: 10.3390/jdb10020023.
7
Context-specific effects of sequence elements on subcellular localization of linear and circular RNAs.序列元件对线性和环状 RNA 亚细胞定位的上下文特异性影响。
Nat Commun. 2022 May 5;13(1):2481. doi: 10.1038/s41467-022-30183-0.
8
FMRP regulates mRNAs encoding distinct functions in the cell body and dendrites of CA1 pyramidal neurons.FMRP 调控在 CA1 锥体神经元胞体和树突中具有不同功能的 mRNAs。
Elife. 2021 Dec 23;10:e71892. doi: 10.7554/eLife.71892.
9
Neuron-Specific IMP2 Overexpression by Synapsin Promoter-Driven AAV9: A Tool to Study Its Role in Axon Regeneration.突触结合蛋白启动子驱动的腺相关病毒 9 介导神经元特异性 IMP2 过表达：研究其在轴突再生中作用的工具。
Cells. 2021 Oct 5;10(10):2654. doi: 10.3390/cells10102654.
10
Crystallin gene expression: Insights from studies of transcriptional bursting.晶体蛋白基因表达：转录爆发研究的新见解。
Exp Eye Res. 2021 Jun;207:108564. doi: 10.1016/j.exer.2021.108564. Epub 2021 Apr 21.
本文引用的文献
1
MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear Organization of Transcription by RNA Editing.MS2-TRIBE可评估蛋白质-RNA相互作用以及RNA编辑对转录的核组织的影响。
iScience. 2020 Jul 24;23(7):101318. doi: 10.1016/j.isci.2020.101318. Epub 2020 Jun 28.
2
The structural basis for RNA selectivity by the IMP family of RNA-binding proteins.IMP 家族 RNA 结合蛋白的 RNA 选择性的结构基础。
Nat Commun. 2019 Sep 30;10(1):4440. doi: 10.1038/s41467-019-12193-7.
3
Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes.利用可扩散探针进行多重和高通量神经元荧光成像。
Nat Commun. 2019 Sep 26;10(1):4377. doi: 10.1038/s41467-019-12372-6.
4
Spatial transcriptome profiling by MERFISH reveals subcellular RNA compartmentalization and cell cycle-dependent gene expression.MERFISH 技术进行空间转录组分析揭示了细胞内 RNA 区室化和细胞周期依赖性基因表达。
Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19490-19499. doi: 10.1073/pnas.1912459116. Epub 2019 Sep 9.
5
Proximity RNA Labeling by APEX-Seq Reveals the Organization of Translation Initiation Complexes and Repressive RNA Granules.APEX-Seq 介导的临近 RNA 标记揭示了翻译起始复合物和抑制性 RNA 颗粒的组织方式。
Mol Cell. 2019 Aug 22;75(4):875-887.e5. doi: 10.1016/j.molcel.2019.07.030.
6
Atlas of Subcellular RNA Localization Revealed by APEX-Seq.细胞内 RNA 定位图谱通过 APEX-Seq 揭示。
Cell. 2019 Jul 11;178(2):473-490.e26. doi: 10.1016/j.cell.2019.05.027. Epub 2019 Jun 20.
7
β-Actin mRNA interactome mapping by proximity biotinylation.通过邻近生物素化绘制 β-肌动蛋白 mRNA 相互作用组图谱。
Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12863-12872. doi: 10.1073/pnas.1820737116. Epub 2019 Jun 12.
8
Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH.基于 RNA seqFISH 的转录组尺度超分辨组织成像
Nature. 2019 Apr;568(7751):235-239. doi: 10.1038/s41586-019-1049-y. Epub 2019 Mar 25.
9
The travels of mRNAs in neurons: do they know where they are going?mRNA 在神经元中的运输：它们知道自己要去哪里吗？
Curr Opin Neurobiol. 2019 Aug;57:110-116. doi: 10.1016/j.conb.2019.01.016. Epub 2019 Feb 19.
10
Linking RNA Sequence, Structure, and Function on Massively Parallel High-Throughput Sequencers.在大规模平行高通量测序仪上连接 RNA 序列、结构和功能。
Cold Spring Harb Perspect Biol. 2019 Oct 1;11(10):a032300. doi: 10.1101/cshperspect.a032300.
Zotero 插件