Biswas Jeetayu, Nunez Leti, Das Sulagna, Yoon Young J, Eliscovich Carolina, Singer Robert H
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Cold Spring Harb Symp Quant Biol. 2019;84:1-10. doi: 10.1101/sqb.2019.84.039396. Epub 2020 Feb 21.
The fate of an RNA, from its localization, translation, and ultimate decay, is dictated by interactions with RNA binding proteins (RBPs). β-actin mRNA has functioned as the classic example of RNA localization in eukaryotic cells. Studies of β-actin mRNA over the past three decades have allowed understanding of how RBPs, such as ZBP1 (IGF2BP1), can control both RNA localization and translational status. Here, we summarize studies of β-actin mRNA and focus on how ZBP1 serves as a model for understanding interactions between RNA and their binding protein(s). Central to the study of RNA and RBPs were technological developments that occurred along the way. We conclude with a future outlook highlighting new technologies that may be used to address still unanswered questions about RBP-mediated regulation of mRNA during its life cycle, within the cell.
RNA的命运，从其定位、翻译到最终降解，都由与RNA结合蛋白（RBP）的相互作用所决定。β-肌动蛋白mRNA一直是真核细胞中RNA定位的经典例子。在过去三十年里，对β-肌动蛋白mRNA的研究使人们了解了诸如ZBP1（IGF2BP1）等RBP如何控制RNA的定位和翻译状态。在这里，我们总结了对β-肌动蛋白mRNA的研究，并重点关注ZBP1如何作为理解RNA与其结合蛋白之间相互作用的模型。RNA和RBP研究的核心是在此过程中出现的技术发展。我们最后展望未来，强调可能用于解决关于细胞内RBP介导的mRNA生命周期调控中仍未解答问题的新技术。
