Petersen G, Hall J C, Rosbash M
Department of Biology, Brandels University, Waltham, MA 02254.
EMBO J. 1988 Dec 1;7(12):3939-47. doi: 10.1002/j.1460-2075.1988.tb03280.x.
We have analyzed and compared the circadian locomotor activity rhythms of Drosophila melanogaster and D.pseudoobscura. The rhythms of D.pseudoobscura are stronger and the periods shorter than those of D.melanogaster. We have also transformed D.melanogaster flies with a hybrid gene containing the coding region of the D.pseudoobscura period (per) gene. Behavioral assays of flies containing this hybrid gene show that the per protein encoded by the D.pseudoobscura per gene is able to rescue the rhythmic deficiencies of arrhythmic, pero1 D.melanogaster. More important, the rhythms of some of these strains are stronger and the periods shorter than those of D.melanogaster (and those of transformants which carry the equivalent D.melanogaster per gene construct) and hence resemble those of D.pseudoobscura. The results suggest that the primary amino acid sequence of the per gene encodes species-specific behavioral instructions that are detectable when only the per gene is transferred to a different species.
我们已经分析并比较了黑腹果蝇和拟暗果蝇的昼夜运动活动节律。拟暗果蝇的节律比黑腹果蝇的更强，周期更短。我们还用一个含有拟暗果蝇周期（per）基因编码区的杂交基因转化了黑腹果蝇。对含有这种杂交基因的果蝇进行的行为分析表明，拟暗果蝇per基因编码的per蛋白能够挽救无节律的pero1黑腹果蝇的节律缺陷。更重要的是，其中一些品系的节律比黑腹果蝇（以及携带等效黑腹果蝇per基因构建体的转化体）的更强，周期更短，因此类似于拟暗果蝇的节律。结果表明，per基因的一级氨基酸序列编码了物种特异性的行为指令，当仅将per基因转移到不同物种时，这些指令是可检测到的。
