果蝇周期基因的种间比较揭示了大片非保守编码DNA。
Interspecific comparison of the period gene of Drosophila reveals large blocks of non-conserved coding DNA.
作者信息
Colot H V, Hall J C, Rosbash M
机构信息
Department of Biology, Brandels University, Waltham, MA 02254.
出版信息
EMBO J. 1988 Dec 1;7(12):3929-37. doi: 10.1002/j.1460-2075.1988.tb03279.x.
Abstract
We have cloned and sequenced the coding region of the period (per) gene from Drosophila pseudoobscura and D. virilis. A comparison with that of D. melanogaster reveals that the conceptual translation products consist of interspersed blocks of conserved and non-conserved amino acid sequence. The non-conserved portion, comprising approximately 33% of the protein sequence, includes the perfect Thr-Gly repeat of D. melanogaster, which is absent from the D. pseudoobscura and D. virilis proteins. Based on these observations and cross-species transformation experiments, we suggest that the interspecific variability in the per primary amino acid sequence contributes to the control of species-specific behaviors.
摘要
我们已经克隆并测序了拟暗果蝇和粗壮果蝇的周期（per）基因的编码区。与黑腹果蝇的编码区进行比较后发现，概念上的翻译产物由保守和非保守氨基酸序列的穿插块组成。非保守部分约占蛋白质序列的33%，其中包括黑腹果蝇特有的完美苏氨酸-甘氨酸重复序列，而在拟暗果蝇和粗壮果蝇的蛋白质中则不存在。基于这些观察结果和跨物种转化实验，我们认为per原代氨基酸序列中的种间变异性有助于控制物种特异性行为。
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