关于巨噬细胞激活与结核杆菌细胞内破坏之间关系的组织化学研究。
Histochemical studies relating the activation of macrophages to the intracellular destruction of tubercle bacilli.
作者信息
Ando M, Dannenberg A M, Sugimoto M, Tepper B S
出版信息
Am J Pathol. 1977 Mar;86(3):623-34.
Dermal tuberculous lesions, both primary and those of reinfection, were produced in rabbits with 14C-labeled BCG and biopsied once at various times. Macrophage activation was evaluated by the indolyl histochemical test for beta-galatosidase, the number of bacilli in macrophages by acid-fast staining, and the breakdown of bacilli by autoradiography. After the rabbits became tuberculin positive, the stongly activated macrophage population contained a) fewer parasitized cell, b) fewer bacilli in each parasitized cell, and c) more "free" 14C-label (not associated with intact bacilli) than the weakly activated macrophage population. These results suggest that the more highly activated macrophages had destroyed many of the bacilli that they once contained and that their power to do so was enhanced by immunologic mechanisms.
用14C标记的卡介苗在兔身上产生原发性和再感染性皮肤结核病变，并在不同时间进行一次活检。通过β-半乳糖苷酶的吲哚基组织化学试验评估巨噬细胞活化，通过抗酸染色评估巨噬细胞内的杆菌数量，通过放射自显影评估杆菌的分解情况。在兔结核菌素呈阳性后，与活化较弱的巨噬细胞群体相比，活化强烈的巨噬细胞群体含有：a）被寄生的细胞较少；b）每个被寄生细胞内的杆菌较少；c）“游离”的14C标记（与完整杆菌无关）较多。这些结果表明，活化程度较高的巨噬细胞已经破坏了它们曾经含有的许多杆菌，并且免疫机制增强了它们这样做的能力。
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