Tepper B S
J Bacteriol. 1968 May;95(5):1713-7. doi: 10.1128/jb.95.5.1713-1717.1968.
A basic difference was found in the kinetics of uptake and utilization of glucose and glycerol by washed suspensions of Mycobacterium phlei. With glucose, the rates of uptake, respiration, and assimilation were saturated at low external substrate concentration. With glycerol, these rates were found to increase with increasing substrate concentration and did not show saturation at any concentration tested. Qualitatively similar patterns were observed for cells grown on either glycerol or glucose. Above a saturation concentration, ratios of cell (14)C to CO(2) (14)C for uniformly labeled (14)C-glucose were constant at a value of 0.96. Glycerol-U-(14)C, on the other hand, yielded cell-(14)C/CO(2)-(14)C ratios which were highest at the lowest glycerol concentration tested, and decreased with increasing substrate concentration. The distribution of the glucose and glycerol carbons assimilated into M. phlei were qualitatively similar. Quantitatively, however, the uptake and assimilation of glycerol was far more rapid than that of glucose for all substrate concentrations employed. These quantitative differences in the utilization of glycerol and glucose could account for the increased content of nonessential lipid and polysaccharide found in glycerol-grown M. phlei.
在草分枝杆菌洗涤悬浮液对葡萄糖和甘油的摄取及利用动力学方面发现了一个基本差异。对于葡萄糖，摄取、呼吸和同化速率在低外部底物浓度时就达到饱和。对于甘油，这些速率随底物浓度增加而增加，并且在任何测试浓度下都未显示饱和。对于在甘油或葡萄糖上生长的细胞，观察到定性相似的模式。在饱和浓度以上，均匀标记的¹⁴C - 葡萄糖的细胞¹⁴C与CO₂¹⁴C的比率恒定在0.96。另一方面，甘油 - U - ¹⁴C产生的细胞¹⁴C/CO₂¹⁴C比率在测试的最低甘油浓度时最高，并随底物浓度增加而降低。被草分枝杆菌同化的葡萄糖和甘油碳的分布在定性上相似。然而，在所有使用的底物浓度下，甘油的摄取和同化在数量上比葡萄糖快得多。甘油和葡萄糖利用上的这些数量差异可以解释在甘油培养的草分枝杆菌中发现的非必需脂质和多糖含量增加的现象。
