食品添加剂E171（二氧化钛）对植物兵豆（Lens culinaris L.）和洋葱（Allium cepa L.）的遗传毒性
Genotoxicity of the food additive E171, titanium dioxide, in the plants Lens culinaris L. and Allium cepa L.
作者信息
Bellani Lorenza, Muccifora Simonetta, Barbieri Francesco, Tassi Eliana, Ruffini Castiglione Monica, Giorgetti Lucia
机构信息
Department of Life Sciences, University of Siena, via A. Moro 2, 53100, Siena, Italy; Institute of Agricultural Biology and Biotechnology, National Research Council (IBBA-CNR), via G. Moruzzi 1, 56124, Pisa, Italy.
Department of Life Sciences, University of Siena, via A. Moro 2, 53100, Siena, Italy.
出版信息
Mutat Res Genet Toxicol Environ Mutagen. 2020 Jan;849:503142. doi: 10.1016/j.mrgentox.2020.503142. Epub 2020 Jan 21.
E171 (titanium dioxide, TiO), an authorized foods and beverage additive, is also used in food packaging and in pharmaceutical and cosmetic preparations. E171 is considered to be an inert and non-digestible material, not storable in animal tissues, but the possible presence of TiO nanoparticles (NP) may present a risk to human health and the environment. We determined the presence of 15% TiO NP in a commercial E171 food additive product, by electron microscopy. The biological effects of E171 were assessed in Lens culinaris and Allium cepa for the following endpoints: percentage of germination, root elongation, mitotic index, presence of chromosomal abnormalities, and micronuclei. The results indicated low phytotoxicity but dose-dependent genotoxicity. We also observed internalization of TiO NP and ultrastructural alterations in the root systems.
E171（二氧化钛，TiO）是一种已获批准的食品和饮料添加剂，也用于食品包装以及药品和化妆品制剂中。E171被认为是一种惰性且不可消化的物质，不会在动物组织中储存，但二氧化钛纳米颗粒（NP）的可能存在可能对人类健康和环境构成风险。我们通过电子显微镜测定了一种市售E171食品添加剂产品中存在15%的二氧化钛纳米颗粒。针对以下终点指标，在兵豆和洋葱中评估了E171的生物学效应：发芽率、根伸长、有丝分裂指数、染色体异常情况以及微核。结果表明其具有低植物毒性，但具有剂量依赖性遗传毒性。我们还观察到了二氧化钛纳米颗粒的内化以及根系的超微结构改变。
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