Ristea Madalina-Elena, Zarnescu Otilia
Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, R-050095 Bucharest, Romania.
Toxics. 2024 Mar 1;12(3):194. doi: 10.3390/toxics12030194.
Indigo carmine has a variety of uses in foods, textiles, medicine, pharmaceuticals, and cosmetics. There are studies reporting the toxic potential of indigo carmine on human health and the environment. In this study, we investigated the cytogenotoxic effects of indigo carmine using apical root cells of . bulbs were subjected to four treatments with indigo carmine (0.0032, 0.0064, 0.0125, and 0.2 mg/mL) and to ultrapure water as a control. After 5 days, root growth, root length, mitotic index, mitotic inhibition, chromosomal anomalies, and cell morphology were analyzed. According to our results, a decrease in root length and mitotic index was observed at all concentrations of indigo carmine. Additionally, several types of chromosomal abnormalities were observed, such as disturbed metaphase, sticky chain metaphase, anaphase bridge, and laggard chromosomes. Moreover, histological observation indicated that indigo carmine induces alterations in various components of root tip tissue, such as deformation and alteration of the cell wall, progressive condensation of chromatin, shrinkage of the nuclei, and an increase in the number of irregularly shaped nuclei and nuclear fragments. Our results indicate that the tested concentrations of indigo carmine may have toxic effects and raise concerns about its intensive use in many fields.
靛蓝胭脂红在食品、纺织品、医学、制药和化妆品等领域有多种用途。有研究报道了靛蓝胭脂红对人类健康和环境的潜在毒性。在本研究中，我们使用[具体植物名称]的根尖细胞研究了靛蓝胭脂红的细胞遗传毒性作用。将[具体植物名称]的鳞茎用靛蓝胭脂红（0.0032、0.0064、0.0125和0.2毫克/毫升）进行四种处理，并以超纯水作为对照。5天后，分析根生长、根长度、有丝分裂指数、有丝分裂抑制、染色体异常和细胞形态。根据我们的结果，在所有浓度的靛蓝胭脂红下均观察到根长度和有丝分裂指数下降。此外，还观察到几种类型的染色体异常，如中期紊乱、粘链中期、后期桥和落后染色体。此外，组织学观察表明，靛蓝胭脂红会诱导根尖组织的各种成分发生改变，如细胞壁变形和改变、染色质逐渐凝聚、细胞核收缩以及不规则形状细胞核和核碎片数量增加。我们的结果表明，所测试浓度的靛蓝胭脂红可能具有毒性作用，并引发了对其在许多领域大量使用的担忧。
