Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
Biochem Pharmacol. 2020 May;175:113868. doi: 10.1016/j.bcp.2020.113868. Epub 2020 Feb 20.
Deoxynivalenol (DON) is the most common mycotoxin in grains, and DON exposure causes gastrointestinal inflammation and systemic immunosuppression. The immunosuppression caused by DON has raised serious concerns about whether it is safe to use probiotics in immunocompromised hosts. Gut microbiota remodeling by Lactobacillus is a potential effective strategy to prevent DON exposure. The athymic nude mice were chose as the model of immunocompromised animals. We tested the effect of the probiotic Lactobacillus rhamnosus GG (LGG) or Lactobacillus acidophilus (LA) supplementation on host protection against DON exposure and the underlying mechanisms in nude mice. DON exposure induced endoplasmic reticulum (ER) stress and impaired intestinal barrier function and microbiota, which were relieved by LGG supplementation but not LA supplementation. LGG supplementation significantly enhanced the intestinal barrier function, increased the body weight and the survival rate in nude mice that exposed to DON for two weeks. Furthermore, LGG supplementation modulated the gut microbiota by increasing the abundance of Bacteroidetes and the levels of the butyrate-producing genes But and Buk to promote butyrate production. Butyrate inhibited the IRE1α/XBP1 signaling pathway to reduce DON-induced intestine injury. In conclusion, LGG supplementation modulated the gut microbiota to promote butyrate production, protecting against DON exposure in nude mice. Both LGG and butyrate show promise for use in protecting against DON exposure.
脱氧雪腐镰刀菌烯醇（DON）是谷物中最常见的霉菌毒素，DON 暴露会引起胃肠道炎症和全身免疫抑制。DON 引起的免疫抑制引起了人们的严重关注，即在免疫功能低下的宿主中使用益生菌是否安全。通过乳杆菌重塑肠道微生物群是预防 DON 暴露的一种潜在有效策略。我们选择无胸腺裸鼠作为免疫功能低下动物的模型。我们测试了益生菌鼠李糖乳杆菌 GG（LGG）或嗜酸乳杆菌（LA）补充对宿主对抗 DON 暴露的保护作用及其在裸鼠中的潜在机制。DON 暴露诱导内质网（ER）应激和损害肠道屏障功能和微生物群，LGG 补充缓解了这些作用，但 LA 补充没有缓解。LGG 补充显著增强了肠道屏障功能，增加了暴露于 DON 两周的裸鼠的体重和存活率。此外，LGG 补充通过增加拟杆菌门的丰度和丁酸盐产生基因 But 和 Buk 的水平来调节肠道微生物群，从而促进丁酸盐的产生。丁酸盐抑制 IRE1α/XBP1 信号通路，减少 DON 诱导的肠道损伤。总之，LGG 补充通过调节肠道微生物群来促进丁酸盐的产生，从而保护裸鼠免受 DON 暴露的影响。LGG 和丁酸盐都有望用于预防 DON 暴露。
