Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100190, China.
Nitric Oxide. 2020 May 1;98:1-19. doi: 10.1016/j.niox.2020.02.002. Epub 2020 Feb 21.
Nitric oxide (NO) is an important signaling molecule implicated in numerous physiological and pathological conditions. NO homeostasis is crucial to its proper functions and misregulation of NO production is implicated in a large number of pathologies. The effect of NO depends on the cell microenvironment as well as its concentration, spatial, and temporal constraints. Thus, the visualization and monitoring of NO in living systems would be valuable for understanding the origin, activities, and biological effects of NO. This review focuses on organic small-molecule fluorescent probes for selective detection and imaging of endogenous NO in vitro and in vivo. The progress suggests that fluorescence imaging is a vital and rapidly technology for real-time detection of NO in biological system.
一氧化氮（NO）是一种重要的信号分子，涉及许多生理和病理状况。NO 的动态平衡对其正常功能至关重要，而 NO 生成的失调与许多病理状况有关。NO 的作用取决于细胞微环境及其浓度、空间和时间限制。因此，在活系统中可视化和监测 NO 将有助于理解 NO 的起源、活性和生物学效应。本文重点介绍用于体外和体内选择性检测和成像内源性 NO 的有机小分子荧光探针。这方面的进展表明，荧光成像技术是实时检测生物体系中 NO 的一种重要且快速发展的技术。
