通过在氯化钠浓度升高的培养基中孵育细胞来改善聚乙二醇诱导的细胞融合。
The improvement of polyethylene glycol-induced cell fusion by incubation of cells in a medium with increased NaCl concentration.
作者信息
Kajstura J, Korohoda W
机构信息
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
出版信息
Folia Histochem Cytobiol. 1988;26(2):77-82.
Incubation of cells in a medium with NaCl concentration increased by 0.2 mol/l for one hour prior to and one hour after the polyethylene glycol induced cell fusion improves the performance of fusion. This procedure increases the percentage of fused cells, the formation of massive fusion products (in case of fusion performed in suspension) and allows the improvement of cell viability. The possible mechanisms involved in such effects of increased NaCl concentration are discussed.
在聚乙二醇诱导细胞融合之前一小时和之后一小时，将细胞置于NaCl浓度增加0.2 mol/l的培养基中孵育一小时，可提高融合效果。该程序可提高融合细胞的百分比、大量融合产物的形成（如果融合是在悬浮液中进行），并可提高细胞活力。文中讨论了NaCl浓度增加产生这种效果可能涉及的机制。
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