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高效液相色谱法和毛细管电泳法结合红-绿-蓝模型同时检测运动饮料中的食用色素和防腐剂。

Simultaneous quantification of food colorants and preservatives in sports drinks by the high performance liquid chromatography and capillary electrophoresis methods evaluated using the red-green-blue model.

机构信息

Jagiellonian University in Kraków, Faculty of Chemistry, Department of Analytical Chemistry, Gronostajowa St. 2, 30-387 Kraków, Poland.

出版信息

J Chromatogr A. 2020 Jun 7;1620:460976. doi: 10.1016/j.chroma.2020.460976. Epub 2020 Feb 14.

Abstract

Consumption of sports drinks is generally associated with a healthy lifestyle, despites a common addition of colorants and preservatives to these products. This work presents the novel methods based on high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), aimed at the simultaneous quantification of both these groups of additives within a single separation. The results obtained show that colorants are generally added in low and rather safe amounts, whereas preservatives (benzoate and sorbate) are much more abundant, and likely pose a potential risk, especially for children. The overall potential of the HPLC and CE methods was critically assessed and compared using the RGB additive color model, a user-friendly tool that allows one to point out and encode particular capabilities and general potential of a method using colors, and to express its holistic effectiveness with one integrated quantitative parameter from 0 to 100%. As a result, the potential predispositions of both methods were delineated.

摘要

尽管在这些产品中通常会添加色素和防腐剂,但运动饮料的消费通常与健康的生活方式有关。本工作提出了基于高效液相色谱 (HPLC) 和毛细管电泳 (CE) 的新方法,旨在在单次分离中同时定量这两类添加剂。所得结果表明,色素的添加量通常较低且相当安全,而防腐剂(苯甲酸和山梨酸)则更为丰富,可能存在潜在风险,尤其是对儿童而言。使用 RGB 添加剂颜色模型对 HPLC 和 CE 方法的整体潜力进行了批判性评估和比较,这是一种用户友好的工具,可用于指出和编码方法的特定能力和一般潜力,并使用从 0 到 100%的一个集成定量参数来表示其整体有效性。结果,描绘了两种方法的潜在倾向。

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