高效离子色谱法测定饮料中的八种合成食用色素
Determination of eight synthetic food colorants in drinks by high-performance ion chromatography.
作者信息
Chen Q C, Mou S F, Hou X P, Riviello J M, Ni Z M
机构信息
Research Center for Eco-Environmental Sciences, Academia Sinica, Beijing, China.
出版信息
J Chromatogr A. 1998 Dec 4;827(1):73-81. doi: 10.1016/s0021-9673(98)00759-6.
Eight synthetic food colorants (Amaranth, Brilliant Blue, Indigo Carmine, New Red, Ponceau 4R, Sunset Yellow, Tartrazine, Allura Red) were determined by high-performance ion chromatography on an anion-exchange analytical column with very low hydrophobicity and visible absorbance detection. Gradient elution with hydrochloric acid-acetonitrile effected both the chromatographic separation of these colorants and the on-line clean-up of the analytical column, which was very advantageous for routine analysis. High-performance ion chromatography may be a solution to the chromatographic analysis for some water-soluble, organic analytes with strong hydrophobicity. The method has been applied to the determination of colorants in drinks and in instant drink powder. No time-consuming pretreatment, as used in conventional liquid chromatography, was needed.
采用具有极低疏水性的阴离子交换分析柱和可见吸光度检测的高效离子色谱法测定了8种合成食用色素（苋菜红、亮蓝、靛蓝胭脂红、新红、胭脂红4R、日落黄、柠檬黄、诱惑红）。用盐酸 - 乙腈进行梯度洗脱，既实现了这些色素的色谱分离，又实现了分析柱的在线净化，这对常规分析非常有利。高效离子色谱法可能是某些具有强疏水性的水溶性有机分析物色谱分析的一种解决方案。该方法已应用于饮料和速溶饮料粉中色素的测定。无需像传统液相色谱法那样进行耗时的预处理。
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