Departments of Experimental and Clinical Medicine, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Experimental and Clinical Biomedical Science, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Anim Reprod Sci. 2020 Sep;220:106314. doi: 10.1016/j.anireprosci.2020.106314. Epub 2020 Feb 14.
Development of in vitro reproduction techniques has not only offered some infertile couples the possibility to have a child, it also revolutionized animal reproduction. Although in vitro reproduction techniques for humans or domestic and non-domestic animals have been designed to mimic in vivo conditions, modifications due to environmental effects or in vitro manipulation of gametes and embryos are unavoidable. For male gametes, in vitro manipulations include techniques to select spermatozoa, cryopreservation and other incubation procedures, during which spermatozoa may be exposed to oxidative stress and other insults that may damage their functions and DNA. The aim of this review is to provide an overview of key studies reporting sperm damage during in vitro manipulation, with particular focus on effects on DNA integrity, a fundamental factor for fertilization and transmission of paternal genetic information to offspring.
体外繁殖技术的发展不仅为一些不孕夫妇提供了生育的可能性，也彻底改变了动物繁殖。尽管用于人类或家畜和非家畜的体外繁殖技术旨在模拟体内条件，但由于环境影响或体外操作配子和胚胎，不可避免地会发生改变。对于精子而言，体外操作包括选择精子的技术、冷冻保存和其他孵育程序，在此过程中，精子可能会受到氧化应激和其他损伤的影响，从而损害其功能和 DNA。本综述的目的是提供一份概述报告，重点介绍体外操作过程中精子损伤的关键研究，特别关注对 DNA 完整性的影响，这是受精和父系遗传信息传递给后代的基本因素。
