School of Medicine, Department of Medical Biochemistry, Koc University, Rumelifeneri Yolu, Sariyer 34450, Istanbul, Turkey; Koc University Research Center for Translational Medicine (KUTTAM), Sariyer 34450, Istanbul, Turkey.
Biochemistry Division, Chemistry Department, Faculty of Science, Erciyes University, 38039 Kayseri, Turkey.
J Biosci Bioeng. 2020 Jun;129(6):679-686. doi: 10.1016/j.jbiosc.2020.01.008. Epub 2020 Feb 21.
Nanoflowers are recently developed flower-shaped nanoparticles consisting of several layers of petals to improve surface reaction and stability. Among them organic-inorganic hybrid nanoflowers have been spotlighted, since they can overcome enzyme-related limitations, including low stability, high production cost, substrate/product inhibition and difficult recovery. In this study, triple enzyme-inorganic hybrid nanoflowers (TrpE@ihNFs) were synthesized using a mixture of three enzymes (α-amylase, lipase and protease) and Cu ions. TrpE@ihNFs were characterized by their morphology and chemical point of view by using different techniques including SEM, FTIR, EDX, and XRD. Afterwards we compared the enzyme activity and stability of TrpE@ihNFs with the free enzymes, including lipase, amylase and protease at the different pH and temperatures spectrophotometrically. Our data reveal that enzyme activities and stability of TrpE@ihNFs were significantly higher compared to the each free enzyme. In conclusion, we showed TrpE@ihNFs which can be used for the treatment of wastewater, biosensors, biocatalysts, and bio-related devices in the future.
纳米花是最近开发的具有多层花瓣的花状纳米粒子，旨在提高表面反应和稳定性。其中，有机-无机杂化纳米花备受关注，因为它们可以克服与酶相关的限制，包括低稳定性、高生产成本、底物/产物抑制和难以回收。在这项研究中，使用三种酶（α-淀粉酶、脂肪酶和蛋白酶）和 Cu 离子的混合物合成了三酶-无机杂化纳米花（TrpE@ihNFs）。通过 SEM、FTIR、EDX 和 XRD 等不同技术对 TrpE@ihNFs 的形态和化学特性进行了表征。然后，我们通过分光光度法比较了 TrpE@ihNFs 与游离酶（包括脂肪酶、淀粉酶和蛋白酶）在不同 pH 值和温度下的酶活性和稳定性。我们的数据表明，与每种游离酶相比，TrpE@ihNFs 的酶活性和稳定性显著更高。总之，我们展示了 TrpE@ihNFs，它们可在未来用于处理废水、生物传感器、生物催化剂和生物相关设备。
